Expression of the arrowhead proteinase inhibitor B (API) gene in the silkworm larvae and pupae
文献类型: 外文期刊
第一作者: Ji, P
作者: Ji, P;Zhang, ZF;He, JL;Wu, XF;Lam, PHY
作者机构:
关键词: arrowhead proteinase inhibitor B gene;gene expression;recombinant BmNPV
期刊名称:ACTA BIOCHIMICA ET BIOPHYSICA SINICA ( 影响因子:3.848; 五年影响因子:3.67 )
ISSN: 0582-9879
年卷期: 1997 年 29 卷 1 期
页码:
收录情况: SCI
摘要: The arrowhead proteinase inhibitor B(API) gene was cloned into baculovirus transfer vectors of pBacPAK8 and pOSX, and two recombinant transfer vectors, pBacPAK (API) and pOSX(API), were constructed. Coinfection was accomplished with either one of the recombinant transfer vectors and Bm-BacPAK6 DNA in BmN cells. Then recombinant virus of CrBK9, CrBK10 and EX was selected. API gene was successfully expressed in silkworm larvae and pupae. The recombinant API(rAPI) showed strong inhibitive activity on bovine pancreas trypsin. The inhibiting activity of the expression product was 1.0 x 10(5)u/ml haemolymph for silkworm larvae and 1.3 x 10(5)u/ml haemolymph for pupae. In addition, SDS-PACE of the preliminarily purified rAPI showed its molecular weight to be about 19kD.
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