Seroprevalence, Isolation, Genotyping, and Pathogenicity of Toxoplasma gondii Strains from Sheep in China

文献类型： 外文期刊

第一作者： Yang, YuRong

作者： Yang, YuRong;Feng, YongJie;Lu, YaoYao;Liang, HongDe;Zhang, LongXian;Yao, QiuXia;Zhu, XingQuan;Wang, YingHua

作者机构：

关键词： Toxoplasma gondii;seroepidemiology;isolation;genotype;virulence;sheep;China

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Toxoplasma gondli is an important cause of reproductive failure in small ruminants that also poses a risk to consumers who consume undercooked meat. However, little is known about sheep toxoplasmosis in China for the world. Therefore, this study was conducted to assess the prevalence of T gondii infection in sheep from China, to isolate T gondii via bioassay in mice and to evaluate the virulence of the isolated T gondii based on vero cell invasion and mice. A total of 840 samples (304 unfrozen hearts and 536 sera) from sheep in China were collected from 2014 to 2016. Heart samples (n = 36) of T gondii seropositive sheep (MAT, >= 25) were bioassayed in mice individually. DNA derived from cell cultured tachyzoites of the isolated T gondii was characterized by PCR-RFLP of 10 loci (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico). The virulence of the T gondii was evaluated based on the mortality and encystation in mice, as well as their growth characteristics in cell culture. Antibodies to T gondii were found in 174 of 840 (20.71%, 304 hearts juice and 536 sera) sheep by the modified agglutination test (cut-off 1:25). Viable T gond!i was isolated from the hearts of two of 36 seropositive sheep hearts. Both genotypes of the sheep heart isolates were ToxoDB#9. The virulence of the two ToxoDB#9 isolations varied significantly. To the best of our knowledge, this is the first report of isolation of ToxoDB#9 strain of T gondii from sheep in China.

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