Transcriptome of High-Sucrose Sugarcane Variety GT35

文献类型: 外文期刊

第一作者: Gao, Yi-Jing

作者: Gao, Yi-Jing;Gui, Yi-Yun;Chen, Zhong-Liang;Qin, Cui-Xian;Wang, Miao;Li, Yang-Rui;Liao, Qing;Li, Yang-Rui;Yang, Li-Tao

作者机构:

关键词: Sugarcane;Transcriptome;Solexa sequencing;Gene expression

期刊名称:SUGAR TECH ( 影响因子:1.591; 五年影响因子:1.688 )

ISSN: 0972-1525

年卷期: 2016 年 18 卷 5 期

页码:

收录情况: SCI

摘要: Sugarcane (Saccharum officinarum L.) is the most important sugar crop and a major source of bio-ethanol. Sucrose accumulation is important in sugarcane, but it has not been investigated by transcriptome analysis. In this study, the transcriptome of a high-sucrose sugarcane variety, GT35, was sequenced using high-throughput Solexa technology. A total of 34,105,138 high-quality reads with an average length of 75 bp were obtained. These reads were assembled into 101,255 unigenes, with a mean length of 460 bp and an N50 length of 640 bp. BLAST searches indicated that 36,472 (36.0 %) unigenes had homologous sequences in the NCBI non-redundant protein sequences databases, and 23,811 (23.5 %) had homologous sequences in the Swiss-Prot databases. A total of 14,613 unigenes were assigned gene ontology terms, and 13,231 were assigned functional annotations and grouped into 25 functional categories. A KEGG pathway analysis of 30,756 unigenes revealed more than 30 pathways in the sugarcane transcriptome. A total of 3420 simple sequence repeats were identified in 3185 unigenes. The data in this study enriched the sugarcane transcriptome resources and will be useful for further comparison and functional genomic studies in genus Saccharum and family Poaceae, and will be useful for further detailed elucidation of sucrose accumulation in sugarcane.

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