Transposable elements cause the loss of self-incompatibility in citrus

文献类型： 外文期刊

第一作者： Hu, Jianbing

作者： Hu, Jianbing;Liu, Chenchen;Du, Zezhen;Guo, Furong;Song, Dan;Wang, Nan;Jiang, Jingdong;Cao, Zonghong;Shi, Chunmei;Zhang, Siqi;Zhu, Chenqiao;Larkin, Robert;Lin, Zongcheng;Xu, Qiang;Ye, Junli;Deng, Xiuxin;Chai, Lijun;Hu, Jianbing;Liu, Chenchen;Du, Zezhen;Larkin, Robert;Lin, Zongcheng;Xu, Qiang;Deng, Xiuxin;Chai, Lijun;Wei, Zhuangmin;Chen, Peng;Bosch, Maurice;Franklin-Tong, Vernonica

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关键词： Self-incompatibility; citrus; S-RNase; S-locus; MITE; evolution

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.8； 五年影响因子：13.2 ）

ISSN： 1467-7644

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Self-incompatibility (SI) is a widespread prezygotic mechanism for flowering plants to avoid inbreeding depression and promote genetic diversity. Citrus has an S-RNase-based SI system, which was frequently lost during evolution. We previously identified a single nucleotide mutation in S-m-RNase, which is responsible for the loss of SI in mandarin and its hybrids. However, little is known about other mechanisms responsible for conversion of SI to self-compatibility (SC) and we identify a completely different mechanism widely utilized by citrus. Here, we found a 786-bp miniature inverted-repeat transposable element (MITE) insertion in the promoter region of the FhiS(2)-RNase in Fortunella hindsii Swingle (a model plant for citrus gene function), which does not contain the S-m-RNase allele but are still SC. We demonstrate that this MITE plays a pivotal role in the loss of SI in citrus, providing evidence that this MITE insertion prevents expression of the S-RNase; moreover, transgenic experiments show that deletion of this 786-bp MITE insertion recovers the expression of FhiS(2)-RNase and restores SI. This study identifies the first evidence for a role for MITEs at the S-locus affecting the SI phenotype. A family-wide survey of the S-locus revealed that MITE insertions occur frequently adjacent to S-RNase alleles in different citrus genera, but only certain MITEs appear to be responsible for the loss of SI. Our study provides evidence that insertion of MITEs into a promoter region can alter a breeding strategy and suggests that this phenomenon may be broadly responsible for SC in species with the S-RNase system.

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