Antimicrobial activity of a novel moricin-like peptide from the Chinese oak silkworm against Vibrio pathogens in sea cucumbers

文献类型： 外文期刊

第一作者： Ye, Bo

作者： Ye, Bo;Li, Shilei;Fan, Qi;Zhao, Zhenjun;Li, Peipei;Yue, Dongmei;Wang, Xuda;Dong, Ying;Liu, Danni;Zhou, Zunchun

作者机构：

关键词： Antimicrobial peptides; Vibrio pathogens; AnpeNPV bacmid system; Sea cucumber; Aquaculture

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.7； 五年影响因子：3.8 ）

ISSN： 2352-5134

年卷期： 2024 年 35 卷

页码：

收录情况： SCI

摘要： Antimicrobial peptides (AMPs) from natural sources represent promising candidates for novel antimicrobial agents instead of antibiotics in aquaculture. Moricin, a family of AMPs from Lepidoptera, has shown bactericidal activity against both gram-positive and gram-negative bacteria. However, it remains uncertain whether these peptides possess antimicrobial activity against Vibrio pathogens affecting various aquatic animals, including sea cucumbers. In this study, we investigated five moricin-like peptides (MLPs) from Antheraea pernyi (Anpe) and identified Anpe-MLP1 as a new member of the moricin family through bioinformatics analysis. Anpe-MLP1 was heterologously expressed using the AnpeNPV bacmid system and purified from A. pernyi pupae. Our findings revealed that recombinant Anpe-MLP1 (rAnpe-MLP1) exhibited antimicrobial activity against gram-positive Staphylococcus aureus (S. aureus ATCC 6538 P), gram-negative Escherichia coli (E. coli ATCC 25922) and three Vibrio species. Moreover, both in vitro and in vivo assays showed that rAnpe-MLP1 effectively inhibited wild-type Vibrio fluvialis infections in the sea cucumber (Apostichopus japonicus). These results suggest that Anpe-MLP1 holds great potential as a novel anti-infective agent and provide valuable insights for its future application in aquaculture.

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