Dissection and validation of a QTL cluster linked toRht-B1locus controlling grain weight in common wheat (Triticum aestivumL.) using near-isogenic lines

文献类型： 外文期刊

第一作者： Guan, Panfeng

作者： Guan, Panfeng;Shen, Xueyi;Mu, Qing;Wang, Yongfa;Wang, Xiaobo;Chen, Yongming;Zhao, Yue;Mao, Weiwei;Guo, Yiwen;Xin, Mingming;Hu, Zhaorong;Yao, Yingyin;Ni, Zhongfu;Sun, Qixin;Peng, Huiru;Guan, Panfeng;Chen, Xiyong;Zhao, Aiju

作者机构：

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

ISSN： 0040-5752

年卷期： 2020 年 133 卷 9 期

页码：

收录情况： SCI

摘要： Key message This study dissected and validated a QTL cluster associated with thousand grain weight on chromosome 4B using multiple near-isogenic lines in common wheat. Grain size and weight are crucial components of wheat yield. Previously, we identified a QTL cluster for thousand grain weight (TGW) on chromosome 4B using the Nongda3338 (ND3338)/Jingdong6 (JD6) doubled haploid population. Here, near-isogenic lines (NILs) in the ND3338 background were developed to dissect and validate the QTL cluster. Based on six independent BC(3)F(3:4)heterogeneous inbred families, the 4B QTL cluster was divided into two linked QTL intervals (designated 4B.1 and 4B.2 QTL). For the 4B.1 QTL, theRht-B1gene, of whichRht-B1ballele reduces plant height (PH) by 21.18-29.34 cm (34.34-53.71%), was demonstrated to be the most likely candidate gene with pleiotropic effects on grain size and TGW. For the 4B.2 QTL, the NIL(JD6)consistently showed an increase in TGW of 3.51-7.68 g (8.84-22.77%) compared with NIL(ND3338)across different field trials, along with a significant increase in PH of 2.26-6.71 cm (3.92-12.01%). Moreover, both QTL intervals had a larger effect on grain width than on grain length. Additionally, the first significant difference in 100-grain fresh weight and 100-grain dry weight between the NIL pairs of the 4B.1 QTL interval (Rht-B1) was observed at 6 days after pollination (DAP), while the differences were first visible at 30 DAP for the 4B.2 QTL interval. Collectively, our work provides a new example of QTL dissection for grain weight in wheat and lays a foundation for further map-based cloning of the major QTL that have potential applications in wheat molecular breeding for high yield.

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