Detection of Japanese encephalitis virus in mosquitoes from Xinjiang during next-generation sequencing arboviral surveillance

文献类型： 外文期刊

第一作者： Hameed, Muddassar

作者： Hameed, Muddassar;Khan, Sawar;Xu, Jinpeng;Zhang, Junjie;Wang, Xin;Di, Di;Anwar, Muhammad Naveed;Wahaab, Abdul;Ma, Xiaochun;Nawaz, Mohsin;Liu, Ke;Li, Beibei;Shao, Donghua;Qiu, Yafeng;Wei, Jianchao;Ma, Zhiyong;Chen, Zheng

作者机构：

关键词： Japanese encephalitis virus; metagenomic next-generation sequencing; mosquito; surveillance

期刊名称：TRANSBOUNDARY AND EMERGING DISEASES （ 影响因子：5.005； 五年影响因子：4.622 ）

ISSN： 1865-1674

年卷期：

页码：

收录情况： SCI

摘要： A total of 548 mosquitoes were collected from different animal farms located near to highly populated cities in Xinjiang and were subjected to metagenomic next-generation sequencing (mNGS). The mNGS data demonstrated that 18,842 (XJ1 strain) and 1,077 (XJ2 strain) of Japanese encephalitis virus (JEV)-related reads were detected in XJ1 and XJ2 mosquito samples collected from Wushi and Wensu counties of Aksu area, which accounted for 0.032% and 0.006% of the total clean reads generated from XJ1 and XJ2 samples, respectively. The Bayesian molecular phylogenetic analysis suggested that XJ1 and XJ2 strains belonged to JEV genotype III and were clustered with JEV strains isolated in China. Notably, Bayesian molecular time line phylogeny revealed that XJ1 strain shared its MRCA with JEV GSS strain about 67 YA, suggesting that XJ1 strain likely originated from linages closely related to GSS strain and spread to Xinjiang later. Overall, these findings suggest that Xinjiang was probably not free from JEV, and thus, a further surveillance of JEV is required in Xinjiang.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序