文献类型： 外文期刊
第一作者： Xu, Jing
作者： Xu, Jing;Wang, Bo;Wang, Ming-Qing;Gao, Jian-Jie;Li, Zhen-Jun;Tian, Yong-Sheng;Peng, Ri-He;Yao, Quan-Hong
作者机构：
关键词： methyl parathion; multigene metabolic engineering; methyl-parathion degradation; genetically engineered bacteria; synthetic biology
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：6.064； 五年影响因子：6.843 ）
ISSN：
年卷期： 2022 年 13 卷
页码：
收录情况： SCI
摘要： Organophosphate compounds are widely used in pesticides to control weeds, crop diseases, and insect pests. Unfortunately, these synthetic compounds are hazardous and toxic to all types of living organisms. In the present work, Escherichia coli was bioengineered to achieve methyl parathion (MP) degradation via the introduction of six synthetic genes, namely, opdS, pnpAS, pnpBS, pnpCS, pnpDS, and pnpES, to obtain a new transformant, BL-MP. MP and its subsequent decomposition intermediates were completely degraded by this transformant to enter the metabolites of multiple anabolic pathways. The MP-degraded strain created in this study may be a promising candidate for the bioremediation of MP and potential toxic intermediates.
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