Resistance gene Ty-1 restricts TYLCV infection in tomato by increasing RNA silencing

文献类型： 外文期刊

第一作者： Ma, Xiaofang

作者： Ma, Xiaofang;Wu, Liming;Ma, Xiaofang;Zhou, Yijun;Ma, Xiaofang;Moffett, Peter

作者机构：

关键词： RNA silencing; Nicotiana benthamiana; Ty-1; RDRs proteins; TYLCV V2

期刊名称：VIROLOGY JOURNAL （ 影响因子：3.8； 五年影响因子：3.7 ）

ISSN：

年卷期： 2024 年 21 卷 1 期

页码：

收录情况： SCI

摘要： A major antiviral mechanism in plants is mediated by RNA silencing through the action of DICER-like (DCL) proteins, which cleave dsRNA into discrete small RNA fragments, and ARGONAUTE (AGO) proteins, which use the small RNAs to target single-stranded RNA. RNA silencing can also be amplified through the action of RNA-dependent RNA polymerases (RDRs), which use single stranded RNA to generate dsRNA that in turn is targeted by DCL proteins. As a counter-defense, plant viruses encode viral suppressors of RNA silencing (VSRs) that target different components in the RNA silencing pathway. The tomato Ty-1 gene confers resistance to the DNA virus tomato yellow leaf curl virus (TYLCV) and has been reported to encode an RDR gamma protein. However, the molecular mechanisms by which Ty-1 controls TYLCV infection, including whether Ty-1 is involved in RNA silencing, are unknown. Here, by using a transient expression assay, we have confirmed that Ty-1 shows antiviral activity against TYLCV in Nicotiana benthamiana. Also, in transient expression-based silencing assays, Ty-1 augmented systemic transgene silencing in GFP transgenic N. benthamiana plants. Furthermore, co-expression of Ty-1 or other RDR gamma proteins from N. benthamiana or Arabidopsis with various proteins resulted in lower protein expression. These results are consistent with a model wherein Ty-1-mediated resistance to TYLCV is due, at least in part, to an increase in RNA silencing activity.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序