Development of an indirect ELISA using a novel linear epitope at the C-terminal region of the VP2 protein to specifically detect antibodies against Senecavirus A

文献类型： 外文期刊

第一作者： Ma, Zhongyuan

作者： Ma, Zhongyuan;Lv, Jianliang;Zhang, Zhongwang;Pan, Li

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关键词： Diagnosis; Senecavirus A; VP2; Epitope; ELISA

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.8； 五年影响因子：3.9 ）

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年卷期： 2022 年 19 卷 1 期

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收录情况： SCI

摘要： Background: Senecavirus A (SVA) is a pathogen that has recently caused porcine idiopathic vesicular disease (PIVD). The clinical signs are similar to those of foot-and-mouth disease, porcine vesicular disease, and vesicular stomatitis. Therefore, identification of SVA as a cause of PIVD is important to eliminate this emerging pathogen. Methods: In this study, an indirect ELISA based on the VP2 epitope (VP2-epitp-ELISA) was developed to detect antibodies directed against SVA. Results: A novel linear epitope ((271)GLRNRFTTGTDEEQ(284)) was first identified at the C-terminus of the VP2 protein by epitope mapping. The diagnostic performance of VP2-epitp-ELISA was estimated by testing a panel of known background sera from swine. Under the optimum test conditions, when the cutoff value was 37%, the diagnostic sensitivity (Dn) and diagnostic specificity (Dp) of the assay were 91.13% and 91.17%, respectively. The accuracy of VP2-epitp-ELISA was validated and further compared with that of commercial diagnostic kits. The diagnostic results showed that VP2-epitp-ELISA did not cross-react with serum positive for other idiopathic vesicular diseases and had a concordance rate of 90.41% with the Swinecheck((R)) SVA bELISA. Conclusions: These results indicate that VP2-epitp-ELISA is suitable for specific detection of antibodies against SVA in swine.

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