文献类型： 外文期刊
第一作者： Li, Peipei
作者： Li, Peipei;Li, Menghang;Yue, Dongmei;Li, Peipei;Chen, Haixia;Li, Menghang
作者机构：
关键词： diagnostic polymerase chain reaction; magnetic nanoparticles; magnetic-free separation; nucleic acid absorbent; solid-phase extraction
期刊名称：JOURNAL OF SEPARATION SCIENCE （ 影响因子：3.645； 五年影响因子：2.943 ）
ISSN： 1615-9306
年卷期：
页码：
收录情况： SCI
摘要： The separation and purification of biomacromolecules such as nucleic acid is a perpetual topic in separation processes and bioengineering (fine chemicals, biopharmaceutical engineering, diagnostics, and biological characterization). In principle, the solid-phase extraction for nucleic acid exhibits efficient phase separation, low pollution risk, and small sample demand, compared to the conventional liquid-phase extraction. Herein, solid-phase extraction methods are systematically reviewed to outline research progress and explore additional solid-phase sorbents and devices for novel, flexible, and high-efficiency nucleic acid separation processes. The functional materials capture nucleic acid, magnetic and magnetic-free solid-phase extraction methods, separation device design and optimization, and high-throughput automatable applications based on high-performance solid-phase extraction are summarized. Finally, the current challenges and promising topics are discussed.
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