Organic selenium compounds determination in the complex matrix by the 1H-77Se HMBC method

文献类型： 外文期刊

第一作者： Wang, Tongtong

作者： Wang, Tongtong;Zhao, Yue;Wang, Min;Zhou, Jian;Yang, Mengrui;Na, Shiyue;Wang, Tongtong;Zhao, Yue;Wang, Min;Zhou, Jian;Yang, Mengrui;Na, Shiyue;Zhao, Yue

作者机构：

关键词： Organic selenium compounds; Quantitative NMR method; Selenium-enriched yeast; Complex matrix; 1H-77Se HMBC

期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：5.304； 五年影响因子：4.723 ）

ISSN： 0026-265X

年卷期： 2022 年 183 卷

页码：

收录情况： SCI

摘要： The development of the organic Selenium (Se) speciation assay to evaluate Se-enriched yeasts (SeYs) with high selectivity and good sensitivity is highly desired. We investigated contents of three organic Se compounds with a proton-detected 1H-77Se multiple-bond correlation (1H-77Se HMBC) method in a complex yeast matrix. The method showed quantitative correlation peaks resulting by the long-range coupling (nJ(77Se,1H), n = 2, 3) between 1H and 77Se atoms. Broadband excitation and homonuclear coupling evolution suppression can be achieved by utilizing the shaped pulse in the 77Se dimension and the constant time period, respectively. Several NMR methods were compared to improve performances of the 1H-77Se HMBC method in sensitivity. The limit of quantifications (LOQs) were 0.10, 0.11, and 0.15 mmol/L for SeMet, MeSeCys and SeCys2, respectively. Furthermore, the method can greatly raise the resolution without any separation technique compared to other one-and two-dimensional NMR and chromatographic method. The validation of the methodology showed satisfactory specificity, accuracy, precision, and linearity. The method was suitable for routine analysis, quality control and market supervision of Se species in the enriched-Se yeast.

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