Functional expression and characterization of Eehinococcus granulosus thioredoxin peroxidase suggests a role in protection against oxidative damage

文献类型： 外文期刊

第一作者： Li, J

作者： Li, J;Zhang, WB;Loukas, A;Lin, RY;Ito, A;Zhang, LH;Jones, M;McManus, DP

作者机构：

关键词： Echinococcus granulosus;thioredoxin peroxidase;thiol-dependent peroxidase activity;antioxidant;H2O2 tolerance;peroxiredoxin gene superfamily

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2004 年 326 卷

页码：

收录情况： SCI

摘要： A full-length cDNA sequence coding for Echinococcus granulosus thioredoxin peroxidase (EgTPx) was isolated from a sheep strain protoscolex cDNA library by immunoscreening using a pool of sera from mice infected with oncospheres. EgTPx expressed as a fusion protein with glutathione S-transferase (GST) exhibited significant thiol-dependent peroxidase activity that protected plasmid DNA from damage by metal-catalyzed oxidation (MCO) in vitro. Furthermore, the suggested antioxidant role for EgTPx was reinforced in an in vivo assay, whereby its expression in BL21 bacterial cells markedly increased the tolerance and survival of the cells to high concentrations of H2O2 compared with controls. Immunolocalization studies revealed that EgTPx was specifically expressed in all tissues of the protoscolex and brood capsules. Higher intensity of labelling was detected in many, but not all, calcareous corpuscle cells in protoscoleces. The purified recombinant EgTPx protein was used to screen sera from heavily infected mice and patients with confirmed hydatid infection. Only a portion of the sera reacted positively with the EgTPx-GST fusion protein in Western blots, suggesting that EgTPx may form antibody-antigen complexes or that responses to the EgTPx antigen may be immunologically regulated. Recombinant EgTPx may prove useful for the screening of specific inhibitors that could serve as new drugs for treatment of hydatid disease. Moreover, given that TPx from different parasitic phyla were phylogenetically distant from host TPx molecules, the development of antiparasite TPx inhibitors that do not react with host TPx might be feasible. (C) 2003 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]Molecular cloning, expression profiles and characterization of a glutathione reductase in Hevea brasiliensis. Deng, Zhi,Zhao, Manman,Liu, Hui,Wang, Yuekun,Li, Dejun,Zhao, Manman.

[2]Excretory/secretory proteome of 14-day schistosomula, Schistosoma japonicum. Cao, Xiaodan,Fu, Zhiqiang,Han, Qian,Lu, Ke,Li, Hao,Zhu, Chuangang,Hong, Yang,Lin, Jiaojiao,Lin, Jiaojiao,Zhang, Min,Han, Yanhui.

[3]Solid lipid nanoparticle suspension enhanced the therapeutic efficacy of praziquantel against tapeworm. Xie, Shuyu,Pan, Baoliang,Wang, Ming,Zhou, Wenzhong,Shi, Baoxin,Zhang, Zhuangzhi,Zhang, Xu. 2011

[4]Genome-wide sequencing of small RNAs reveals a tissue-specific loss of conserved microRNA families in Echinococcus granulosus. Bai, Yun,Jin, Lei,Kang, Hui,Zhu, Yongqiang,Zhang, Lu,Li, Xia,Ma, Fengshou,Wang, Shengyue,Zhang, Zhuangzhi,Zhao, Li,Shi, Baoxin,Li, Jun,Zhang, Wenbao,McManus, Donald P.. 2014

[5]Further studies on an intermediate host murine model showing that a primary Echinococcus granulosus infection is protective against subsequent oncospheral challenge. Zhang, WB,You, H,Zhang, ZZ,Turson, G,Hasyet, A,McManus, DP.

[6]Epidemiology and control of echinococcosis in central Asia, with particular reference to the People's Republic of China. Zhang, Wenbao,Li, Jun,Wen, Hao,Zhang, Wenbao,Li, Jun,McManus, Donald P.,Zhang, Zhuangzhi,Shi, Baoxin,Wu, Weiping,Zhou, Xiaonong. 2015

[7]Cloning and characterization of an Echinococcus granulosus ecdysteroid hormone nuclear receptor HR3-like gene. Yang, Mei,Zhang, Fuchun,Li, Jun,Wang, Hui,Guo, Baoping,Wu, Chuanchuan,Shou, Xi,Yang, Ning,Zhang, Wenbao,Yang, Mei,Wu, Jun,Zhang, Zhuangzhi,McManus, Donald P.. 2017

[8]Immunoglobulin profiles in a murine intermediate host model of resistance for Echinococcus granulosus infection. Zhang, WB,You, H,Li, J,Zhang, ZZ,Turson, G,Aili, H,Wang, JC,McManus, DP. 2003

[9]A gene family from Echinococcus granulosus differentially expressed in mature adult worms. Zhang, WB,Li, J,You, H,Zhang, ZZ,Turson, G,Loukas, A,McManus, DP. 2003

[10]Genetic variation of the 8-kDa glycoprotein family from Echinococcus granulosus, Taenia multiceps and Taenia hydatigena. Jia Wan-zhong,Yan Hong-bin,Lou Zhong-zi,Ni Xing-wei,Liu Hong-xia,Li Hong-min,Guo Ai-jiang,Fu Bao-quan. 2011

[11]Vaccination of bovines against Echinococcus granulosus (cystic echinococcosis). Heath, David D.,Robinson, Christine,Shakes, Trevor,Huang, Yan,Gulnur, Tursun,Shi, Baoxing,Zhang, Zhuangzhi,Anderson, Garry A.,Lightowlers, Marshall W..

[12]Effect of irradiation and storage time on lipid oxidation of chilled pork. Cheng, Anwei,Xu, Tongcheng,Du, Fangling,Wang, Wenliang,Zhu, Qingjun,Wan, Fachun. 2011

[13]Antioxidant and tyrosinase inhibitory activity of Rosa roxburghii fruit and identification of main bioactive phytochemicals by UPLC-Triple-TOF/MS. Zeng, Fangfang,Limwachiranon, Jarukitt,Li, Li,Feng, Simin,Luo, Zisheng,Ge, Zhiwei,Wang, Yansheng.

[14]Antioxidative and Hepatoprotective Activities of Deinoxanthin-Rich Extract from Deinococcus radiodurans R1 against Carbon Tetrachloride-Induced Liver Injury in Mice. Cheng, Jianhui,Zheng, Zhiguo,Tian, Bing,Hua, Yuejin,Cheng, Jianhui,Zhang, Zuofa,Lv, Guoying,Zheng, Zhiguo. 2014

[15]Physiological Mechanism of Enhancing Salt Stress Tolerance of Perennial Ryegrass by 24-Epibrassinolide. Wu, Wenli,Zhang, Qiang,Yang, Zhiping,Ervin, Erik. H.,Zhang, Xunzhong. 2017

[16]Molecular characterization of Babesia microti thioredoxin (BmTrx2) and its expression patterns induced by antiprotozoal drugs. Huang, Jingwei,Xiong, Kang,Zhang, Houshuang,Zhao, Yanzhen,Cao, Jie,Gong, Haiyan,Zhou, Yongzhi,Zhou, Jinlin. 2018

[17]Protective Effects of Blueberry Anthocyanins against H2O2-Induced Oxidative Injuries in Human Retinal Pigment Epithelial Cells. Huang, Wu-Yang,Wu, Han,Li, Da-Jing,Song, Jiang-Feng,Xiao, Ya-Dong,Liu, Chun-Quan,Zhou, Jian-Zhong,Huang, Wu-Yang,Sui, Zhong-Quan. 2018

[18]ANTI-FATIGUE EFFECTS OF POLYPHENOLS EXTRACTED FROM ARECA CATECHU L. HUSK AND DETERMINATION OF THE MAIN COMPONENTS BY HIGH PERFORMANCE CAPILLARY ELECTROPHORESIS. Tang, Minmin,Chen, Hua,Zhao, Songlin,Wang, Hui,Qi, Jing. 2016

[19]Antioxidant and Anti-aging Activities of &ITSilybum Marianum&IT Protein Hydrolysate in Mice Treated with D-galactose. Zhu Shu Yun,Yang Jing,Zhou Yue,Zhu Shu Yun,Jiang Ning,Tu Jie. 2017

[20]Ferulic acid: A natural compound as an efficient feed additive for GIFT (Oreochromis niloticus). Yu, L-J,Wu, F.,Jiang, M.,Yang, C-G,Liu, W.,Tian, J.,Lu, X.,Wen, H.,Yu, L-J. 2018

作者其他论文 更多>>

微信小程序