Effects of Lead on the Morphology and Structure of the Nucleolus in the Root Tip Meristematic Cells of Allium cepa L.

文献类型： 外文期刊

第一作者： Jiang, Ze

作者： Jiang, Ze;Zhang, Huaning;Qin, Rong;Zou, Jinhua;Wang, Junran;Shi, Qiuyue;Jiang, Wusheng;Liu, Donghua;Zhang, Huaning;Qin, Rong

作者机构：

关键词： Allium cepa var. agrogarum L.;lead (Pb);nucleolus;nucleophosmin;nucleolin;fibrillarin

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2014 年 15 卷 8 期

页码：

收录情况： SCI

摘要： To study the toxic mechanisms of lead (Pb) in plants, the effects of Pb on the morphology and structure of the nucleolus in root tip meristematic cells of Allium cepa var. agrogarum L. were investigated. Fluorescence labeling, silver-stained indirect immunofluorescent microscopy and western blotting were used. Fluorescence labeling showed that Pb ions were localized in the meristematic cells and the uptake and accumulation of Pb increased with treatment time. At low concentrations of Pb (1-10 mu M) there were persistent nucleoli in some cells during mitosis, and at high concentration (100 mu M) many of the nucleolar organizing regions were localized on sticky chromosomes in metaphase and anaphase cells. Pb induced the release of particles containing argyrophilic proteins to be released from the nucleus into the cytoplasm. These proteins contained nucleophosmin and nucleolin. Pb also caused the extrusion of fibrillarin from the nucleus into the cytoplasm. Western blotting demonstrated the increased expression of these three major nucleolar proteins under Pb stress.

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