Temporally integrated transcriptome analysis reveals ASFV pathology and host response dynamics

文献类型： 外文期刊

第一作者： Lv, Lin

作者： Lv, Lin;Lv, Lin;Zhang, Tianyun;Jia, Hanying;Ahsan, Asif;Zhao, Xiaoyang;Shen, Ning;Zhang, Yanyan;Chen, Teng;Shen, Zhiqiang;Shen, Zhiqiang;Shen, Ning

作者机构：

关键词： ASFV; transcriptome; host-virus interaction; inflammatory; immune response; pathogenicity

期刊名称：FRONTIERS IN IMMUNOLOGY （ 影响因子：8.786； 五年影响因子：8.876 ）

ISSN： 1664-3224

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： African swine fever virus (ASFV) causes a lethal swine hemorrhagic disease and is currently responsible for widespread damage to the pig industry. The pathogenesis of ASFV infection and its interaction with host responses remain poorly understood. In this study, we profiled the temporal viral and host transcriptomes in porcine alveolar macrophages (PAMs) with virulent and attenuated ASFV strains. We identified profound differences in the virus expression programs between SY18 and HuB20, which shed light on the pathogenic functions of several ASFV genes. Through integrated computational analysis and experimental validation, we demonstrated that compared to the virulent SY18 strain, the attenuated HuB20 quickly activates expression of receptors, sensors, regulators, as well as downstream effectors, including cGAS, STAT1/2, IRF9, MX1/2, suggesting rapid induction of a strong antiviral immune response in HuB20. Surprisingly, in addition to the pivotal DNA sensing mechanism mediated by cGAS-STING pathway, infection of the DNA virus ASFV activates genes associated with RNA virus response, with stronger induction by HuB20 infection. Taken together, this study reveals novel insights into the host-virus interaction dynamics, and provides reference for future mechanistic studies of ASFV pathogenicity.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序