Short communication. Enhancement of the immune responses to vaccination against foot-and-mouth disease in mice by oral administration of Quillaja saponaria-A and extracts of Cochinchina momordica seed

文献类型: 外文期刊

第一作者: Xiao, C. W.

作者: Xiao, C. W.;Bao, G.;Rajput, Z. I.;Hu, S. H.;Rajput, Z. I.;Soomro, N. A.

作者机构:

关键词: adjuvant;ELISA;oral immunization;vaccine

期刊名称:SPANISH JOURNAL OF AGRICULTURAL RESEARCH ( 影响因子:1.238; 五年影响因子:1.731 )

ISSN: 1695-971X

年卷期: 2013 年 11 卷 1 期

页码:

收录情况: SCI

摘要: This study was designed to evaluate the effects of oral administration of extracts from Cochinchina momordica seed (ECMS) or Quillaja saponaria-A (Quil-A) on the immune responses in mice immunized with foot and mouth disease virus (FMDV)-serotype O vaccine. Forty-two imprinting control region (ICR) mice were randomly divided into seven groups of 6 animals in each group, and a dose of 400 mu g of Quil-A or ECMS was orally administered for 1, 2 or 3 days. After that, the animals were subcutaneously immunized twice with FMD vaccine at 3-week intervals and blood samples were collected 2-weeks after boosting for measurement of FMDV-specific IgG and its subclasses. Spleens were collected for lymphocytes proliferation assay. Results indicated that serum FMDV-specific IgG and the IgG subclass responses were significantly enhanced in mice orally administered ECMS or Quil-A when compared with the control group (p < 0.05). Lymphocytes proliferation response to FMD vaccine was significantly enhanced by ECMS compared with the control (p < 0.05). This study illustrates that ECMS induced immunomodulatory effects and performed better than Quil-A.

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