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大菱鲆AFLP分析体系的建立

文献类型: 中文期刊

作者: 季士治 1 ; 王伟继 2 ; 雷霁霖 2 ; 孔杰 2 ;

作者机构: 1.中国海洋大学生命科学与技术学部

2.农业部海洋渔业资源可持续利用重点开放实验室中国水产科学研究院黄海水产研究所

关键词: AFLP;大菱鲆;反应体系

期刊名称: 海洋水产研究

ISSN: 1000-7075

年卷期: 2007 年 28 卷 01 期

页码: 8-14

收录情况: 北大核心 ; CSCD

摘要: 本研究构建了大菱鲆扩增片段长度多态性(AFLP)分析体系,对DNA提取、双酶切反应、连接反应、预扩增反应、选择性扩增反应和银染等步骤进行了分析。结果表明,用EcoRI/MseI双酶切、核心序列加3个选择性碱基的选择性扩增引物、1∶6(w∶w)的EcoRI端与MseI端选择性扩增引物比和20μl选择性扩增体积,可得到十分稳定的结果。所得产物经电泳和银染后可获得条带清晰、背景干扰小的图像。该体系的构建为AFLP技术在大菱鲆相关研究中的应用奠定了基础。

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