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唇(鱼骨)鳍基因组AFLP反应体系的建立及优化

文献类型: 中文期刊

作者: 佟广香 1 ; 匡友谊 1 ; 尹家胜 1 ; 徐伟 1 ;

作者机构: 1.中国水产科学研究院黑龙江水产研究所

关键词: 唇(鱼骨);AFLP;影响因素

期刊名称: 江西农业大学学报

ISSN: 1000-2286

年卷期: 2009 年 031 卷 004 期

页码: 727-733

收录情况: 北大核心 ; CSCD

摘要: 以唇(鱼骨)为实验材料,通过对扩增片段长度多态性(AFLP)的几个关键技术参数进行研究,建立适合于唇(鱼骨)的AFLP反应体系.结果显示.通过DNA的质量、酶的浓度、酶切时间及扩增时稀释倍数等条件的调整,EcoRI、Tru9I酶切组合和PstI、TaqI酶切组合均能够获得清晰可靠的图谱,二者的结果比较显示,EcoRI、Tru9I酶切组合扩增结果的多态性要稍差于PstI、TaqI酶切组合的扩增结果,为利用AFLP标记对唇(鱼骨)基因组进行深入研究打下基础.

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