A smartphone-assisted high-throughput integrated color-sensing platform for the rapid detection of Campylobacter coli
文献类型： 外文期刊
作者： Lin, Qijie 1 ; Jia, Kaiyuan 2 ; Gou, Hongchao 3 ; He, Haishan 1 ; Wen, Junping 1 ; Shen, Haiyan 3 ; Chen, Kaifeng 1 ; Wu, Yuqian 1 ; Lu, Bijia 1 ; Liao, Ming 1 ; Han, Yuxing 2 ; Zhang, Jianmin 1 ;
作者机构： 1.South China Agr Univ, Coll Vet Med,Key Lab Zoonoses Prevent & Control G, Ctr Emerging & Zoonot Dis,Key Lab Zoonoses,Minist, Natl & Reg Joint Engn Lab Medicament Zoonoses Pre, Guangzhou 510642, Peoples R China
2.South China Agr Univ, Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China
3.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou 510640, Peoples R China
关键词： High-throughput detection; Campylobacter coli; Visual LAMP; HSI color Space
期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.056； 五年影响因子：6.295 ）
ISSN： 0023-6438
年卷期： 2022 年 167 卷
页码：
收录情况： SCI
摘要： Campylobacter is one of the most common foodborne pathogens worldwide. A new smartphone-assisted high-throughput integrated color-sensing platform, called the HICS platform, was developed for the rapid detection of Campylobacter coli. This platform was based on the visual loop-mediated isothermal amplification method. Using this system, as many as 64 samples could be assessed in less than an hour after enrichment. 60 meat samples were tested to compare the performance of the HICS platform and other methods. Having the consistent detection result with TaqMan qPCR (Quantitative Real-time Polymerase Chain Reaction), the HICS platform was able to reliably detect C. coli in meat samples, and its limit of detection is 550 CFU/mL and 120 copies/mu L, which was 10-fold higher than that of the PCR (Polymerase Chain Reaction) method. To conclude, considering that our platform showed robust performance and does not require any expensive equipment, it can also be reliably applied for the high-throughput detection of other pathogens.
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