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Sterol demethylation inhibitor fungicide resistance in Colletotrichum siamense from chili is caused by mutations in CYP51A and CYP51B

文献类型： 外文期刊

作者： Chen, Wenchan 1 ; Wei, Lingling 2 ; Hou, Rongxian 1 ; Zhao, Yangyang 1 ; Zhao, Yancun 1 ; Liu, Fengquan 1 ;

作者机构： 1.Minist Sci & Technol, Inst Plant Protect, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base,Jiangsu Acad Agr Sci, Nanjing 210014, Jiangsu, Peoples R China

2.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

3.Hainan Univ, Coll Plant Protect, Key Lab Green Prevent & Control Trop Plant Dis &, Minist Educ, Haikou 570228, Hainan, Peoples R China

关键词： Anthracnose; Chili; DMI fungicides; Fitness; CYP51A and CYP51B; Resistance mechanism

期刊名称：PHYTOPATHOLOGY RESEARCH （ 影响因子：3.955； 五年影响因子：3.955 ）

ISSN： 2096-5362

年卷期： 2022 年 4 卷 1 期

页码：

收录情况： SCI

摘要： Anthracnose, caused by fungi of the genus Colletotrichum, is a serious disease of chili worldwide. Sterol 14 alpha-demethylation inhibitors (DMIs) are a class of chemical fungicides that can effectively control anthracnose diseases. In this study, 22 Colletotrichum isolates collected from commercial chili fields in Zhangzhou, Fujian Province, China, were identified as Colletotrichum siamense. The sensitivities of the 22 C. siamense isolates to tebuconazole were determined based on the EC50 (50% effective inhibition concentration) value. The results showed that the EC50 values of the two isolates to tebuconazole were 0.039 +/- 0.0036 and 0.042 +/- 0.0012 mg/L, while the other 20 isolates showed significantly decreased sensitivities to tebuconazole, with EC50 values ranging from 0.61 +/- 0.056 to 1.94 +/- 0.11 mg/L. Sequence analysis of CYP51A and CYP51B revealed five genotype mutations (i. e., CYP51A(V46L, D115V, P163S, R306K, E397D), CYP51A(D115V, S164Y, R306K, E397D), CYP51A(D115V, R306K, P339T, E397D), CYP51A(D115V, R306K, E397D, S400N), and CYP51A(D115V, R306K, E397D)CYP51B(R266H)) in the resistant isolates. The tebuconazole-resistant isolates of five genotypes suffered a fitness penalty and exhibited cross-resistance to difenoconazole, prochloraz, and propiconazole. Additionally, the five genotype mutations were validated as being responsible for tebuconazole-resistance in C. siamense by construction of replacement mutants. Overexpression of CYP51A and CYP51B was not detected in the replacement mutants of the five genotypes. Overall, the present study is the first to report DMI resistance in C. siamense and provides significant information for rational use of DMIs to control chili anthracnose.

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