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Molecular characterization of a c-Jun NH2-terminal kinase (JNK)-interacting protein 4 (Lv-JIP4) in Litopenaeus vannamei and its potential role in the Lv-p38(MAPK) pathway in response to low temperature

文献类型： 外文期刊

作者： Zhong, Ping 1 ; Xu, Zhongneng 1 ; Chen, Wei 2 ; Peng, Kai 2 ; Sun, Yuping 2 ; Wu, Xiaopeng 2 ; Sun, Huiming 2 ; Chen, Xiaoying 2 ; Luo, Peng 4 ; Li, Chaozheng 5 ; Li, Huo 6 ; Huang, Wen 1 ;

作者机构： 1.Jinan Univ, Inst Hydrobiol, Guangzhou 510632, Peoples R China

2.Guangdong Acad Agr Sci, Inst Anim Sci, Lab Aquat Sci,Guangdong Key Lab Anim Breeding & N, Key Lab Anim Nutr & Feed Sci South China,Minist A, Guangzhou 510640, Peoples R China

3.Guangdong Ocean Univ, Fishery Coll, Zhanjiang 524088, Peoples R China

4.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, Guangzhou 510301, Peoples R China

5.Lingnan Lab Modern Agr Sci & Technol Guangdong Pr, Maoming Branch, Maoming 525000, Peoples R China

6.Guangdong Jinyang Biotechnol Co LTD, Guangdong Prov Engn & Technol Res Ctr, Maoming 525027, Peoples R China

关键词： Litopenaeus vannamei; C-Jun NH2-terminal kinase (JNK)-interacting proteins 4 (JIP4); Lv-p38(MAPK); Low-temperature stress

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.385； 五年影响因子：3.645 ）

ISSN： 2352-5134

年卷期： 2021 年 21 卷

页码：

收录情况： SCI

摘要： 9C-Jun NH2-terminal kinase (JNK)-interacting protein 4 (JIP4) is an essential molecule that organizes and links components of the mitogen-activated protein kinase (MAPK) pathway. In the present study, a novel JIP4 gene was identified in Litopenaeus vannamei and designated Lv-JIP4. The full-length Lv-JIP4 cDNA was 3923 bp in length and contained a 170-bp 5 '-untranslated region (UTR), a 72-bp 3 '-UTR, and a 3,681-bp open reading frame (ORF). The Lv-JIP4 protein contained three functional domains, including the Jnk-SapK_ap_N, JIP_LZII and WD40 domains. Lv-JIP4 mRNA was broadly distributed in all studied tissues, with the highest level in muscle. The in situ hybridization results showed that the Lv-JIP4 was scattered in muscle fibres and near the sarcolemma. Subcellular localization analysis indicated that Lv-JIP4 was located in the cytoplasm but not in the nucleus. The transcript levels of Lv-JIP4 in muscle were found to be significantly increased by low temperature, low pH, high salinity, poly(I:C), and LPS challenges, indicating that it might play a crucial role in responding to adverse stresses. The transcript levels of Lv-JIP4, Lv-p38a(MAPK9) and Lv-p38b(MAPK) in muscle were significantly upregulated after challenge with low temperature, suggesting that Lv-JIP4 may be the positive regulator of Lv-p38a(MAPK) and Lv-p38b(MAPK) under low temperature stress conditions. In addition, after knockdown of Lv-JIP4 expression, the protein levels of total Lv-p38(MAPK) and phospho-Lv-p38(MAPK) in the experimental dsRNA group were attenuated compared with those in the control group after 48 h (h), 60 h and 72 h of low-temperature stress, indicating that it might activate and phosphorylate the Lv-p38(MAPK) protein in response to cold stress. Here, we elucidated the molecular characteristics and the potential role of Lv-JIP4 and further refined the signal transduction mechanism of L. vannamei in response to low-temperature stress.

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