Resveratrol Protects against Zearalenone-Induced Mitochondrial Defects during Porcine Oocyte Maturation via PINK1/Parkin-Mediated Mitophagy
文献类型： 外文期刊
作者： Xu, Jiehuan 1 ; Sun, Lingwei 1 ; He, Mengqian 1 ; Zhang, Shushan 1 ; Gao, Jun 1 ; Wu, Caifeng 1 ; Zhang, Defu 1 ; Dai, Jianjun 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Sci, Shanghai 201106, Peoples R China
2.Shanghai Municipal Key Lab Agrigenet & Breeding, Shanghai 201106, Peoples R China
3.Shanghai Engn Res Ctr Breeding Pig, Shanghai 201106, Peoples R China
4.Minist Agr & Rural Affairs, Key Lab Livestock & Poultry Resources Pig Evaluat, Shanghai 201106, Peoples R China
关键词： zearalenone; mitophagy; resveratrol; PINK1; Parkin; porcine oocyte
期刊名称：TOXINS （ 影响因子：5.075； 五年影响因子：5.305 ）
ISSN：
年卷期： 2022 年 14 卷 9 期
页码：
收录情况： SCI
摘要： Mitochondria hold redox homeostasis and energy metabolism as a crucial factor during oocyte maturation, while the exposure of estrogenic mycotoxin zearalenone causes developmental incapacity in porcine oocyte. This study aimed to reveal a potential resistance of phytoalexin resveratrol against zearalenone during porcine oocyte maturation and whether its mechanism was related with PTEN-induced kinase 1 (PINK1)/Parkin-mediated mitophagy. Porcine oocytes were exposed to 20 mu M zearalenone with or without 2 mu M resveratrol during in vitro maturation. As for the results, zearalenone impaired ultrastructure of mitochondria, causing mitochondrial depolarization, oxidative stress, apoptosis and embryonic developmental incapacity, in which mitophagy was induced in response to mitochondrial dysfunction. Phytoalexin resveratrol enhanced mitophagy through PINK1/Parkin in zearalenone-exposed oocytes, manifesting as enhanced mitophagy flux, upregulated PINK1, Parkin, microtubule-associated protein light-chain 3 beta-II (LC3B-II) and downregulated substrates mitofusin 2 (MFN2), voltage-dependent anion channels 1 (VDAC1) and p62 expressions. Resveratrol redressed zearalenone-induced mitochondrial depolarization, oxidative stress and apoptosis, and accelerated mitochondrial DNA copy during maturation, which improved embryonic development. This study offered an antitoxin solution during porcine oocyte maturation and revealed the involvement of PINK1/Parkin-mediated mitophagy, in which resveratrol mitigated zearalenone-induced embryonic developmental incapacity.
- 相关文献
作者其他论文 更多>>
-
Isoglycyrrhizin supplementation of frozen goat semen-extender improves post-thaw sperm quality and in vitro fertilization rates
作者：Liu, Fuqin;Sun, Lingwei;Xu, Jiehuan;He, Mengqian;Wu, Caifeng;Gao, Jun;Dai, Jianjun;Liu, Fuqin;Sun, Lingwei;Xu, Jiehuan;He, Mengqian;Wu, Caifeng;Gao, Jun;Dai, Jianjun;Shen, Haoxing;Zhu, Huibin;Luo, Feng
关键词：Shanghai chongming white goat; Semen cryopreservation; Isoglycyrrhizin (ISL); Antioxidant; Oxidative stress; In vitro fertilization
-
Cell Cryopreservation in a Microfluidic Chip With Vision-Based Fluid Control and Region Reaching
作者：Miao, Shu;Jia, Yongyi;Li, Xiang;Jiang, Ze;Xu, Jiehuan
关键词：Microfluidics; Robots; Embryo; Automation; Loading; Visualization; Animals; Microscopy; Manuals; Process control; Automation at micro-nano scales; biological cell manipulation; microfluidic chip
-
Comparative Analysis of Classic Semen Extenders for Frozen-Thawed Boar Semen
作者：Kong, Yuting;Kong, Yuting;He, Mengqian;Gao, Jun;Xu, Jiehuan;Lu, Naisheng;Wu, Caifeng;Sun, Lingwei;Dai, Jianjun;Kong, Yuting;He, Mengqian;Gao, Jun;Xu, Jiehuan;Lu, Naisheng;Wu, Caifeng;Sun, Lingwei;Dai, Jianjun;Dai, Jianjun
关键词：boar semen; cryopreservation; semen extender; energy metabolism; spermatozoa motility
-
Preserving Life: How Retinoic Acid (RA) Enhances Cell Viability and Reduces Apoptosis in Cryopreserved Blastocyst Cells of Pudong Chickens
作者：Sun, Lingwei;Liu, Fuqin;He, Mengqian;Xu, Jiehuan;Wu, Caifeng;Zhagn, Shushan;Gao, Jun;Dai, Jianjun;Sun, Lingwei;Liu, Fuqin;He, Mengqian;Xu, Jiehuan;Wu, Caifeng;Zhagn, Shushan;Gao, Jun;Dai, Jianjun
关键词：retinoic acid (RA); blastoderm cells (BCs); cryopreservation; cell viability; cell adhesion; apoptosis; cell cycle; mitochondrial function
-
Genomic Dissection of Chinese Yangtze River Delta White Goat Based on Whole Genome Sequencing
作者：Gao, Jun;Sun, Lingwei;Liao, Rongrong;Lyu, Yuhua;Zhang, Shushan;Xu, Jiehuan;He, Mengqian;Wu, Caifeng;Zhang, Defu;Lin, Yuexia;Dai, Jianjun;Gao, Jun;Sun, Lingwei;Zhang, Shushan;Xu, Jiehuan;He, Mengqian;Wu, Caifeng;Zhang, Defu;Dai, Jianjun;Gao, Jun;Sun, Lingwei;Zhang, Shushan;Xu, Jiehuan;He, Mengqian;Wu, Caifeng;Zhang, Defu;Dai, Jianjun
关键词：Chinese Yangtze River Delta white goat; whole genome sequencing; selective sweep analysis
-
BGP-15 improves quality of goat sperm by mitigating oxidative stress during cryopreservation
作者：Liu, Fuqin;Zhu, Xing;Dai, Jianjun;Gao, Jun;He, Mengqian;Xu, Jiehuan;Wu, Caifeng;Zhang, Shushan;Sun, Lingwei
关键词：Goat; Sperm; Cryopreservation; Sperm characteristics; Antioxidant; Apoptosis; BGP-15
-
Comparison of Nutritional Flavor Substances in Meat Between Shanghai Local Pig Breeds and Commercial DLY Breed
作者：Shi, Yan;Zhou, Xinli;Shi, Yan;Tu, Weilong;Cao, Mengqian;Sun, Lingwei;Zhang, Shushan;Xu, Jiehuan;He, Mengqian;Wu, Caifeng;Zhang, Defu;Dai, Jianjun;Gao, Jun;Shi, Yan;Cao, Mengqian;Sun, Lingwei;Zhang, Shushan;Xu, Jiehuan;He, Mengqian;Wu, Caifeng;Zhang, Defu;Dai, Jianjun;Gao, Jun;Shi, Yan;Sun, Lingwei;Zhang, Shushan;Xu, Jiehuan;He, Mengqian;Wu, Caifeng;Zhang, Defu;Dai, Jianjun;Gao, Jun;Tu, Weilong;Sun, Lingwei;Zhang, Shushan;Zhang, Defu;Dai, Jianjun;Gao, Jun;Cao, Mengqian
关键词：meat traits; nutritional flavor substances; amino acids; fatty acids; thiamine; inosine monophosphate
