Resveratrol Protects against Zearalenone-Induced Mitochondrial Defects during Porcine Oocyte Maturation via PINK1/Parkin-Mediated Mitophagy
文献类型： 外文期刊
作者： Xu, Jiehuan 1 ; Sun, Lingwei 1 ; He, Mengqian 1 ; Zhang, Shushan 1 ; Gao, Jun 1 ; Wu, Caifeng 1 ; Zhang, Defu 1 ; Dai, Jianjun 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Sci, Shanghai 201106, Peoples R China
2.Shanghai Municipal Key Lab Agrigenet & Breeding, Shanghai 201106, Peoples R China
3.Shanghai Engn Res Ctr Breeding Pig, Shanghai 201106, Peoples R China
4.Minist Agr & Rural Affairs, Key Lab Livestock & Poultry Resources Pig Evaluat, Shanghai 201106, Peoples R China
关键词： zearalenone; mitophagy; resveratrol; PINK1; Parkin; porcine oocyte
期刊名称：TOXINS （ 影响因子：5.075； 五年影响因子：5.305 ）
ISSN：
年卷期： 2022 年 14 卷 9 期
页码：
收录情况： SCI
摘要： Mitochondria hold redox homeostasis and energy metabolism as a crucial factor during oocyte maturation, while the exposure of estrogenic mycotoxin zearalenone causes developmental incapacity in porcine oocyte. This study aimed to reveal a potential resistance of phytoalexin resveratrol against zearalenone during porcine oocyte maturation and whether its mechanism was related with PTEN-induced kinase 1 (PINK1)/Parkin-mediated mitophagy. Porcine oocytes were exposed to 20 mu M zearalenone with or without 2 mu M resveratrol during in vitro maturation. As for the results, zearalenone impaired ultrastructure of mitochondria, causing mitochondrial depolarization, oxidative stress, apoptosis and embryonic developmental incapacity, in which mitophagy was induced in response to mitochondrial dysfunction. Phytoalexin resveratrol enhanced mitophagy through PINK1/Parkin in zearalenone-exposed oocytes, manifesting as enhanced mitophagy flux, upregulated PINK1, Parkin, microtubule-associated protein light-chain 3 beta-II (LC3B-II) and downregulated substrates mitofusin 2 (MFN2), voltage-dependent anion channels 1 (VDAC1) and p62 expressions. Resveratrol redressed zearalenone-induced mitochondrial depolarization, oxidative stress and apoptosis, and accelerated mitochondrial DNA copy during maturation, which improved embryonic development. This study offered an antitoxin solution during porcine oocyte maturation and revealed the involvement of PINK1/Parkin-mediated mitophagy, in which resveratrol mitigated zearalenone-induced embryonic developmental incapacity.
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