Effects of UV/H2O2 degradation and step gradient ethanol precipitation on Sargassum fusiforme polysaccharides: Physicochemical characterization and protective effects against intestinal epithelial injury
文献类型： 外文期刊
作者： Yao, Wanzi 1 ; Liu, Mengyuan 1 ; Chen, Xiaoyong 1 ; You, Lijun 1 ; Ma, Yongxuan 3 ; Hileuskaya, Kseniya 4 ;
作者机构： 1.South China Univ Technol, Sch Food Sci & Engn, 381 Wushan Rd, Guangzhou 510640, Peoples R China
2.Res Inst Food Nutr & Human Hlth 111 Ctr, Guangzhou 510640, Guangdong, Peoples R China
3.Guangdong Acad Agr Sci, Sericultural & Agri Food Res Inst, Guangzhou 510610, Guangdong, Peoples R China
4.Natl Acad Sci Belarus, Inst Chem New Mat, Minsk, BELARUS
关键词： Sargassum fusiforme; Polysaccharides; UV/H2O2; IEC-6 cells; Intestinal barrier function; Intestinal inflammation
期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：7.425； 五年影响因子：7.716 ）
ISSN： 0963-9969
年卷期： 2022 年 155 卷
页码：
收录情况： SCI
摘要： In this study, the degraded purified fraction from Sargassum fusiforme polysaccharides (SFP), named DSFP, was produced by the treatment of ultraviolet/hydrogen peroxide (UV/H2O2) degradation and step gradient ethanol precipitation. Results showed that the treatment significantly reduced the molecular weight of polysaccharides, from 282.83 kDa to 18.54 kDa, and influenced their surface morphology and roughness. SFP and DSFP were typical sulfated polysaccharides, mainly composed of fucose, galacturonic acid, glucuronic acid, galactose, and mannose. Both SFP and DSFP increased cell migration during intestinal epithelial wound healing and stimulated the cell cycle progression by promoting the transition from G0/G1 to S phase in the rat intestine epithelium cells (IEC-6). But DSFP had a stronger positive effect on wound healing and cell migration than SFP. It reinforced the intestinal barrier function and attenuated lipopolysaccharides-induced intestinal inflammation. DSFP significantly downregulated the expression of Toll-like receptor 4, tumor necrosis factor-alpha, interleukin-6, interleukin-1 beta, and inducible nitric oxide synthase by 53.14%, 92.41%, 66.01%, 68.24%, and 78.09%, respectively, and upregulated that of interleukin-10 by 2.48 folds when compared to the model. Therefore, the treatment (UV/H2O2 degradation and step gradient ethanol precipitation) could effectively improve the protective effects against intestinal epithelial injury.
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