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Myostatin increases the expression of matrix metalloproteinase genes to promote preadipocytes differentiation in pigs

文献类型： 外文期刊

作者： Zhu, Zhe 1 ; Ali, Akhtar 1 ; Wang, Jing 1 ; Qi, Shijin 1 ; Hua, Zaidong 1 ; Ren, Hongyan 1 ; Zhang, Liping 1 ; Gu, Hao 1 ; Molenaar, Adrian 1 ; Babar, Masroor Ellahi 5 ; Bi, Yanzhen 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Anim Sci & Vet Med, Key Lab Anim Embryo Engn & Mol Breeding Hubei Pr, Wuhan 430064, Hubei, Peoples R China

2.Virtual Univ Pakistan, Dept Biotechnol, Lahore, Pakistan

3.South Cent Univ National, Coll Life Sci, Wuhan, Peoples R China

4.Rumen Microbiol & Anim Nutr & Physiol AgResearch, Grasslands Campus, Palmerston North, New Zealand

5.Univ Agr Dera Ismail Khan, Dera Ismail Khan, Pakistan

关键词： Myostatin; Matrix; Metalloproteinase; preadipocyte; pig

期刊名称：ADIPOCYTE （ 影响因子：3.553； 五年影响因子：4.657 ）

ISSN： 2162-3945

年卷期： 2022 年 11 卷 1 期

页码：

收录情况： SCI

摘要： Myostatin (MSTN) resulted in reduced backfat thickness in MSTN-knockout (MSTN-KO) pigs, whereas the underlying mechanism remains elusive. In this study, RNA sequencing (RNA-seq) was used to screen differentially expressed genes (DEGs) in porcine fat tissues. We identified 285 DEGs, including 4 adipocyte differentiation-related genes (ADRGs). Matrix Metalloproteinase-2/7 (MMP-2/7), fibronectin (FN), and laminin (LN) were differentially expressed in MSTN-KO pigs compared with wild-type (WT) pigs. To investigate the molecular mechanism, we treated the preadipocytes with siRNA and recombinant MSTN protein. The results indicated that MSTN increased the expression of MMP-2/7/9 and promoted the preadipocyte differentiation. To further validate the effect of MSTN on MMP-2/7/9 expression, we treated MSTN-KO PK15 cells with recombinant MSTN protein and detected the expression of MMP-2/7/9. The data showed that MSTN increases the expression of MMP-2/7/9 in PK15. This study revealed that MSTN promoted preadipocyte differentiation and provided the basis for the mechanism of fatty deposition in pigs.

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