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Identification of a novel mutant allele, fzp-15, involved in panicle branch pattern of rice (Oryza sativa)

文献类型： 外文期刊

作者： Chen, Haiyuan 1 ; Cai, Yue 2 ; Zhang, Suobing 1 ; Tang, Weijie 1 ; Fang, Xianwen 1 ; Zhang, Yunhui 1 ; Yamamotob, Tosh 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Germplasm Resources & Biotechnol, Prov Key Lab Agrobiol, Nanjing 210014, Peoples R China

2.Jiangsu Acad Agr Sci, Lixiahe Dist Agr Sci Inst Jiangsu, Yangzhou, Jiangsu, Peoples R China

3.Yangzhou Univ, Jiangsu Coinnovat Ctr Modern Prod Technol Grain C, Yangzhou, Jiangsu, Peoples R China

关键词： advantageous alleles; CRISPR; FZP; panicle architecture; rice

期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）

ISSN： 0179-9541

年卷期： 2021 年 140 卷 4 期

页码：

收录情况： SCI

摘要： FRIZZY PANICLE (FZP) is involved in the development of panicle branching in rice. Herein, a novel mutant fzp-15, demonstrating the sequential rounds of branching and severe defects in SM (spikelet meristem) formation, was identified. Map-based cloning revealed that the abnormal panicle architecture of fzp-15 resulted from an amino acid substitution (Glu (E) to Ala (A)) in the N-terminal ERF domain of rice FZP protein. To generate new advantageous FZP alleles increasing grain numbers, CRISPR/Cas9 multiplex editing system was used to target the 5' untranslation region (UTR) of FZP in 'Nipponbare' (NIP). The 85-bp (-156 to -72 bp) or 149-bp (-157 to -9 bp) deletion in the upstream region of FZP can significantly increase grain numbers without a decrease in grain size, while the 38-bp deletion (-44 to -7 bp) in the upstream region of FZP has little effect in changing grain numbers. These results indicated that the 113-bp fragment (-157 to -45 bp) upstream of FZP is important to its function and deletion of this region can be used to increase grain numbers in rice.

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