文献类型： 外文期刊
作者： Xiong, Ai-Sheng 1 ; Peng, Ri-He 1 ; Zhuang, Jing 1 ; Gao, Feng 1 ; Li, Yi 3 ; Cheng, Zong-Ming 2 ; Yao, Quan-Hong 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China
2.Univ Tennessee, Dept Plant Sci, Knoxville, TN USA
3.Univ Connecticut, Dept Plant Sci, Storrs, CT 06269 USA
关键词： chemical gene synthesis;PCR;primer design;error correction;application;biotechnology;HIGH-LEVEL EXPRESSION;SITE-DIRECTED MUTAGENESIS;OVERLAP EXTENSION PCR;HETEROLOGOUS PROTEIN-PRODUCTION;HEPATITIS-B-VIRUS;FLANKING HOMOLOGY REGIONS;POLYMERASE-CHAIN-REACTION;IN-VITRO RECOMBINATION;SYNONYMOUS CODON USAGE;TRANSGENIC RICE PLANTS
期刊名称：FEMS MICROBIOLOGY REVIEWS （ 影响因子：16.408； 五年影响因子：18.835 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Chemical synthesis of DNA sequences provides a powerful tool for modifying genes and for studying gene structure, expression and function. Modified genes and consequently protein/enzymes can bridge genomics and proteomics research or facilitate commercial applications of gene and protein technologies. In this review, we will summarize various strategies, designing softwares and error correction methods for chemical gene synthesis, particularly for the synthesis and assembly of long DNA molecules based on polymerase cycling assembly. Also, we will briefly discuss some of the major applications of chemical synthesis of DNA sequences in basic research and applied areas.
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