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Genome Characterization and Phylogenetic Analysis of the First Bovine Rhinitis B Virus Isolate in China

文献类型： 外文期刊

作者： Zhai, Shao-Lun 1 ; Xie, Yi-Lun 1 ; Zhai, Qi 1 ; Wen, Xiao-Hui 1 ; Lv, Dian-Hong 1 ; Chen, Qin-Ling 1 ; Wang, Gang 1 ; Wei 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Minist Agr Rural Affairs, Guangzhou, Peoples R China

2.Guangdong Acad Agr Sci, Key Lab Anim Dis Prevent Guangdong Prov, Inst Anim Hlth, Guangzhou, Peoples R China

3.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangzhou, Peoples R China

关键词： bovine rhinitis B virus; bovine respiratory disease complex; high-throughput sequencing; complete genome; nucleotide deletion; genetic variation

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.412； 五年影响因子：3.588 ）

ISSN：

年卷期： 2021 年 8 卷

页码：

收录情况： SCI

摘要： Bovine rhinitis B virus (BRBV) is an emerging viral species in the genus Aphthovirus, family Picornaviridae. Studies suggested that BRBV was considered a potential etiological agent of bovine respiratory disease complex (BRDC). BRBV has been reported in the United States, Sweden, Canada, Japan, and Mexico. However, little information of BRBV was available in China. In this study, we performed viral metagenomic analysis in a calf with respiratory disease. The results showed high abundance (3.85) of BRBV nucleotide and 248 mapped reads in calf samples. Online BLASTn analysis showed that three contigs of those had the highest nucleotide similarity (95%) with one Swedish BRBV isolate (BRBV_SWE1, GenBank accession no. ). To identify the genome characterization of the Chinese BRBV isolate (designated CHN1), six couples of overlapping RT-PCR primers were designed according to genome sequences of BRBV_SWE1. Through gene cloning and splicing, we obtained the genome information of CHN1, possessing 7,465 nucleotides (46.6% G+C). Although CHN1 had the highest nucleotide similarity (95.1%) with BRBV_SWE1, one 11-nucleotide (ACATTTGTTGT) deletion occurred in the 5' untranslated region compared to SWE1. Phylogenetic analysis showed that CHN1 clustered together with BRBV_SWE1, and far from other BRBV isolates. This study recorded the first discovery of BRBV infection in China. Further investigation should be made in order to evaluate the infection status and epidemiological significance of BRBV in China.

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