Rapid detection of zoonotic Streptococcus suis serotype 2 and 14 by enzyme-activated probe fluorescence quantitative PCR method
文献类型： 外文期刊
作者： Su, Yaxing 1 ; Meng, Jiajia 1 ; Zhao, Mingwei 1 ; Li, Chunling 2 ; Zhai, Shaolun 2 ; Li, Yan 2 ; Chu, Pinpin 2 ; Bian, Zhibiao 2 ; Zhang, Kunli 2 ; Yang, Dongxia 2 ; Jiang, Zhiyong 2 ; Gou, Hongchao 2 ; Xu, Chenggang 1 ;
作者机构： 1.South China Agr Univ, Coll Vet Med, Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China
2.Guangdong Acad Agr Sci, Guangdong Prov Key Lab Livestock Dis Prevent, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Inst Anim Hlth, Guangzhou 510640, Peoples R China
3.Guangdong Lab Lingnan Modern Agr, Maoming Branch, Maoming, Peoples R China
4.Guangdong Prov Key Lab Res Technol Pig Breeding &, Guangzhou 510640, Peoples R China
关键词：
期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.6； 五年影响因子：2.7 ）
ISSN：
年卷期： 2024 年 20 卷 1 期
页码：
收录情况： SCI
摘要： Streptococcus suis serotypes 2 and 14 are the most common zoonotic strains, but previous identification methods made distinguish these two serotypes from other S. suis serotypes difficult. To effectively prevent and control them, there is an urgent need for a highly sensitive and specific method to identify these two serotypes. In this study, a fluorescent probe was designed for the single nucleotide polymorphism site at cpsK 483 of Streptococcus suis type 2 and type 14 compared with other serotypes, and an enzyme-activated probe quantitative PCR (EA-probe qPCR) method was established for the detection of Streptococcus suis type 2 and type 14 by combining with the specific hydrolysis characteristics of the RNase H2 enzyme. The results showed that the optimal probe concentration for this method was 0.5 mu M and the optimal RNase H2 enzyme concentration was 25 mU.This method showed no reactivity with genomic DNA from Streptococcus suis strains 1/2, 5, 7, 9, 23, 28, 29, and 31, confirming its high specificity. And its sensitivity can reach 18.4 CFU. In addition, 19 clinical strains of Streptococcus suis type 2 or type 1/2 were tested. The results showed 100% agreement with the gene sequencing method. In conclusion, this method can meet the needs of accurate laboratory testing of Streptococcus suis serotypes 2 and 14 and has value for clinical prevention.
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