Hypoxia Induces Oxidative Injury and Apoptosis via Mediating the Nrf-2/Hippo Pathway in Blood Cells of Largemouth Bass (Micropterus salmoides)
文献类型： 外文期刊
作者： Xin, Yu 1 ; Yang, Zhixin 2 ; Zhu, Yuke 2 ; Li, Yixuan 2 ; Yu, Jie 2 ; Zhong, Wanqing 2 ; Chen, Yanhan 2 ; Lv, Xiaohui 3 ; Hu, Junru 3 ; Lin, Jinjiang 4 ; Miao, Yutao 2 ; Wang, Lei 2 ;
作者机构： 1.Guangdong Ocean Univ, Coll Fisheries, Zhanjiang, Peoples R China
2.South China Normal Univ, Guangdong Prov Key Lab Healthy & Safe Aquaculture, Sch Life Sci,Guangzhou Key Lab Subtrop Biodivers, Inst Modern Aquaculture Sci & Engn IMASE,Key Lab, Guangzhou, Peoples R China
3.Guangdong Acad Agr Sci, Inst Anim Sci, Guangdong Key Lab Anim Breeding & Nutr, Minist Agr & Rural Affairs,Key Lab Anim Nutr & Fe, Guangzhou, Peoples R China
4.Uyu Guangzhou Technol Co Ltd, Guangzhou, Peoples R China
5.Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai, Peoples R China
关键词： climate warming; hypoxia; Micropterus salmoides; blood cells; Nrf2; Hippo pathway
期刊名称：FRONTIERS IN ECOLOGY AND EVOLUTION （ 影响因子：4.493； 五年影响因子：4.648 ）
ISSN： 2296-701X
年卷期： 2022 年 10 卷
页码：
收录情况： SCI
摘要： Investigating how aquatic animals respond to hypoxia brought about by changes in environmental temperature may be of great significance to avoid oxidative injury and maintain the quality of farmed fish in the background of global warming. Here, we investigated the effects of hypoxia on oxidative injury and environment-sensing pathway in blood cells of Micropterus salmoides. The total blood cell count (TBCC) and Giemsa staining showed that hypoxia could lead to damage of blood cells. Flow cytometry analysis confirmed that the apoptosis rate, Ca2+ level, NO production and ROS of blood cells were significantly increased under hypoxia stress. Environment-sensing pathways, such as Nrf2 pathway showed that hypoxia resulted in significant up-regulation of hiF-1 alpha subunit (Hif-1 alpha), nuclear factor erythroid 2-related factor 2 (Nrf2) and kelch-1ike ECH- associated protein l (Keap1) expression. Meanwhile, the expression of Hippo pathway-related genes such as MOB kinase activator 1 (MOB1), large tumor suppressor homolog 1/2 (Lats1/2), yes-associated protein/transcriptional co-activator with PDZ-binding motif (YAP/TAZ), protein phosphatase 2A (PP2A) were significantly increased in blood cells after hypoxia exposure. In addition, hypoxia stress also increased the expression of catalase (CAT) and glutathione peroxidase (GPx), but decreased the expression of superoxide dismutase (SOD). Consequently, our results suggested that hypoxia could induce oxidative injury and apoptosis via mediating environment-sensing pathway such as Nrf2/Hippo pathway in blood cells of M. salmoides.
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