Integrated approach with UHPLC-Q-Exactive-tandem mass spectrometry, network analysis, and molecular docking to determine potential active compounds and mechanisms of Rhizoma Musae decoction in osteoarthritis treatment
文献类型： 外文期刊
作者： Zhang, Jian 1 ; Shen, Wanyan 2 ; Liu, Fanzhi 1 ; He, Hehe 2 ; Han, Shuquan 1 ; Luo, Lina 1 ;
作者机构： 1.Guizhou Acad Agr Sci, Guizhou Inst Subtrop Crops, Guiyang, Peoples R China
2.Guizhou Weikang Zifan Pharmaceut Co Ltd, Res & Dev Dept, Guiyang, Peoples R China
关键词： Rhizoma Musae decoction; UHPLC-Q-Exactive-MS/MS; network analysis; osteoarthritis; signaling pathways
期刊名称：FRONTIERS IN PHARMACOLOGY （ 影响因子：4.8； 五年影响因子：5.2 ）
ISSN：
年卷期： 2025 年 15 卷
页码：
收录情况： SCI
摘要： Objective This study aimed to identify the potential active compounds in Rhizoma Musae decoction and understand their mechanisms of action in osteoarthritis treatment.Methods UHPLC-Q-Exactive-MS/MS technology was used for an in-depth analysis of the chemical compounds present in Rhizoma Musae decoction. A network analysis approach was used to construct a comprehensive network of compounds, targets, and pathways, which provided insights into the molecular mechanisms of Rhizoma Musae decoction in osteoarthritis treatment.Results The integrated analysis revealed the presence of 534 chemical compounds in Rhizoma Musae decoction, with 7beta-hydroxyrutaecarpine, 7,8-dihydroxycoumarin, pinocembrin diacetate, and scopoletin being identified as potential active compounds. Potential targets such as GAPDH, AKT1, TNF, IL6, and SRC were implicated in key pathways including MAPK signaling pathway, lipid and atherosclerosis, PI3K-Akt signaling pathway, and IL-17 signaling pathway. Molecular docking studies showed significant binding affinity between the core targets and key components. In vitro cell experiments have demonstrated that RM decoction can enhance cell proliferation and upregulates the expression of TNF alpha, IL-6, and SRC, while down-regulating the expression of GAPDH and AKT1.Conclusion The potential active compounds present in Rhizoma Musae decoction influence specific targets and signaling pathways involved in osteoarthritis pathogenesis, providing new insights for the functional development and utilization of RM.
- 相关文献
作者其他论文 更多>>
-
Fracture-healing effects of Rhizoma Musae ethanolic extract: An integrated study using UHPLC-Q-Exactive-MS/MS, network pharmacology, and molecular docking
作者：Zhang, Jian;Liu, Fanzhi;Han, Shuquan;Luo, Lina;Shen, Wanyan;He, Hehe
关键词：
-
Mechanistic insights into nutrient profiles, cellulose, and hemicellulose dynamics in red and green Toona sinensis buds during cold storage
作者：Tan, Guo-Fei;Zhao, Qian;Wang, Fu;Li, Shu-Yao;Liu, Zi-Yu;Zhang, Jian;Tan, Guo-Fei;Zhang, Xin-Qi;Zhong, Xiu-Lai;Zhu, Shun-Hua;Lei, Xiu-Juan;Han, Zhong-Min;Zhang, Jian
关键词：
T. sinensis buds; nutrient component; cold storage; cytological observation; observation; gene expression -
The Recognition and Utilization of Natural Genetic Resources for Advances in Plant Biology through Genomics and Biotechnology
作者：Tan, Guofei;Zhang, Jian;Tan, Guofei;Que, Feng;Zhang, Jian
关键词：
-
Determining rapeseed lodging angles and types for lodging phenotyping using morphological traits derived from UAV images
作者：Wang, Chufeng;Xu, Shijie;You, Yunhao;Zhang, Jian;Liu, Bin;You, Liangzhi;Wang, Chufeng;Xu, Shijie;You, Yunhao;Zhang, Jian;Liu, Bin;Yang, Chenghai;Kuai, Jie;Xie, Jing;Zuo, Qingsong;Yan, Mingli;Du, Hai;Ma, Ni;You, Liangzhi;Wang, Tao;Wu, Hao
关键词：Crop lodging; Total lodging angle; Root lodging angle; Lodging types; Unmanned aerial vehicle
-
Discovery of Pyrido[1,2-α] Pyrimidinone Mesoionic Compounds as Potential Control Agents Against Potato Virus Y
作者：Cui, Honghao;Wu, Zengxue;Zhang, Luoman;Wu, Duanpu;Hu, Deyu;Zhang, Jian;Cui, Honghao
关键词：Potato virus Y; mesoionic; coat protein; interaction; prevention and treatment
-
Construction of a high-density genetic map based on large-scale marker development in Coix lacryma-jobi L. using specific-locus amplified fragment sequencing (slaf-seq)
作者：Yang, Chenglong;Ban, Xiuwen;Zhou, Mingqiang;Zhou, Yu;Luo, Kai;Yang, Xiaoyu;Li, Zhifang;Liu, Fanzhi;Li, Qing;Luo, Yahong;Zhou, Xiang;Lei, Jing;Long, Peilin;Yang, Chenglong;Ban, Xiuwen;Zhou, Mingqiang;Zhou, Yu;Luo, Kai;Yang, Xiaoyu;Li, Zhifang;Liu, Fanzhi;Li, Qing;Luo, Yahong;Zhou, Xiang;Lei, Jing;Long, Peilin;Wang, Jian;Guo, Jianchun;Guo, Jianchun
关键词：Coix lacryma-jobi L.; SLAF-seq; High-density genetic map; SNPs; Linkage group
-
Exploring the action mechanism of Oxalis corniculata L. decoction in treating osteoarthritis utilizing liquid chromatography-mass spectrometry technology combined with network pharmacology
作者：Zhang, Jian;Zhang, Jian;Shen, Wanyan;He, Hehe
关键词：network pharmacology; osteoarthritis; Oxalis corniculata L. decoction; UHPLC-Q-Exactive-MS/MS
