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Rice miR5504 regulates plant height by affecting cell proliferation and expansion

文献类型： 外文期刊

作者： Wang, Huihui 1 ; Ouyang, Jiexiu 1 ; Jian, Wenjia 1 ; Li, Meng 1 ; Zhong, Jiancong 1 ; Yan, Xin 1 ; Gao, Jiadong 2 ; Wang, Xin 1 ; Li, Shaobo 1 ;

作者机构： 1.Nanchang Univ, Sch Life Sci, Key Lab Mol Biol & Genet Engn Jiangxi Prov, Nanchang, Peoples R China

2.Guangdong Key Lab Crop Germplasm Resources Preserv, Guangzhou, Peoples R China

3.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangzhou, Peoples R China

4.Hokkaido Univ, Res Fac Agr, Sapporo, Japan

5.Nanchang Univ, Sch Life Sci, Key Lab ofMolecular Biol & Genet Engn ofJiangxi Pr, Nanchang 330031, Peoples R China

期刊名称：PHYSIOLOGIA PLANTARUM （ 影响因子：6.4； 五年影响因子：5.9 ）

ISSN： 0031-9317

年卷期： 2023 年 175 卷 5 期

页码：

收录情况： SCI

摘要： miRNAs play critical roles in the regulation of plant growth and development by cleaving mRNA or repressing transcription. In our previous study, miR5504 with unknown functions was captured by small RNA sequencing. Here, the function and characters of miR5504 were extensively analyzed using CRISPR/Cas9, overexpression strategy, Northern blot, cytological analysis, and transcriptomics analysis. We found that the dwarf phenotype of mir5504 mutants (mir5504-1 and mir5504-2) appeared on 35-day seedlings and became more apparent at the mature stage. The cytological results showed a substantial decrease in the vascular bundle number, cell number and cell length in the mir5504 mutant compared with NIP. In addition, we found that miR5504 regulated plant height by targeting LOC_Os08g16914. The results of RNA-seq revealed that numerous biological processes were mainly enriched, including DNA-binding transcription factor activity, transferase activity, regulation of transcription, metabolic process, and protein binding. Meanwhile, KEEG analysis showed that numerous proteins were associated with cellular processes and metabolism pathways. Taken together, miR5504 may be involved in the regulation of plant height by affecting cell expansion and division of internode in rice.

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