文献类型： 外文期刊

作者： Matsumoto, Haruna ¹ ; Qian, Yuan ¹ ; Fan, Xiaoyan ¹ ; Chen, Sunlu ⁶ ; Nie, Yanxia ⁷ ; Qiao, Kun ¹ ; Xiang, Dandan ⁸ ; Zhang, Xinzhong ⁹ ; Li, Meng ¹ ; Guo, Bo ¹⁰ ; Shen, Peilin ¹ ; Wang, Qiangwei ¹ ; Yu, Yunlong ¹ ; Cernava, Tomislav ⁴ ; Wang, Mengcen ¹ ;

作者机构： 1.Zhejiang Univ, Inst Pesticide & Environm Toxicol, State Key Lab Rice Biol, Hangzhou 310058, Peoples R China

2.Zhejiang Univ, Inst Pesticide & Environm Toxicol, Minist Agr & Rural Affairs, Lab Mol Biol Crop Pathogens & Insects, Hangzhou 310058, Peoples R China

3.Zhejiang Univ, Coll Agr & Biotechnol, Key Lab Biol Crop Pathogens & Insects Zhejiang Pr, Hangzhou 310058, Peoples R China

4.Graz Univ Technol, Inst Environm Biotechnol, Petersgasse 12, A-8010 Graz, Austria

5.Hokkaido Univ, Grad Sch Agr, Global Educ Program AgriSci Frontiers, Sapporo, Hokkaido 0608589, Japan

6.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

7.Chinese Acad Sci, Ecol & Environm Sci Ctr, South China Bot Garden, Guangzhou 510650, Peoples R China

8.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangzhou 510640, Peoples R China

9.Chinese Acad Agr Sci, Tea Res Inst, Hangzhou 310008, Peoples R China

10.Shanghai Int Studies Univ, Shanghai 200083, Peoples R China

11.Xiaoshan Agr Comprehens Dev Zone & Management Com, Hangzhou 311200, Peoples R China

关键词： Phytopathogen; Virulence factor; Transcriptome reprogramming; Agrochemical; Pesticide; Rice

期刊名称：FUNDAMENTAL RESEARCH （ 影响因子：6.2； 五年影响因子：6.2 ）

ISSN： 2096-9457

年卷期： 2022 年 2 卷 2 期

页码：

收录情况： SCI

摘要： Bacteria equipped with virulence systems based on highly bioactive small molecules can circumvent their host???s defense mechanisms. Pathogens employing this strategy are currently threatening global rice production. In the present study, variations in the virulence of the highly destructive Burkholderia plantarii were observed in different rice-producing regions. The environment-linked variation was not attributable to any known host-related or external factors. Co-occurrence analyses indicated a connection between reduced virulence and 5-Amino-1,3,4-thiadiazole-2-thiol (ATT), a non-bactericidal organic compound. ATT, which accumulates in rice plants during metabolization of specific agrochemicals, was found to reduce virulence factor secretion by B. plantarii up to 88.8% and inhibit pathogen virulence by hijacking an upstream signaling cascade. Detailed assessment of the newly discovered virulence inhibitor resulted in mechanistic insights into positive effects of ATT accumulation in plant tissues. Mechanisms of virulence alleviation were deciphered by integrating high-throughput data, gene knockout mutants, and molecular interaction assays. TroK, a histidine protein kinase in a two-component system that regulates virulence factor secretion, is likely the molecular target antagonized by ATT. Our findings provide novel insights into virulence modulation in an important plant-pathogen system that relies on the host???s metabolic activity and subsequent signaling interference.