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Fitness benefits play a vital role in the retention of the Pi-ta susceptible alleles

文献类型： 外文期刊

作者： Liu, Jia 1 ; Zhang, Suobing 2 ; Xie, Pengfei 1 ; Wang, Long 3 ; Xue, Jia-Yu 1 ; Zhang, Yanmei 1 ; Lu, Ruisen 1 ; Hang, Yueyu 1 ; Wang, Yue 1 ; Sun, Xiaoqin 1 ;

作者机构： 1.Jiangsu Prov & Chinese Acad Sci, Inst Bot, Nanjing 210014, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Crop Germplasm & Biotechnol, Jiangsu Prov Platform Conservat & Utilizat Agr Ge, Nanjing 210014, Peoples R China

3.Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210023, Peoples R China

4.Nanjing Agr Univ, Acad Adv Interdisciplinary Studies, Coll Hort, Nanjing 210095, Peoples R China

关键词： fitness; Pi-ta; the susceptible alleles; knockout

期刊名称：GENETICS （ 影响因子：4.402； 五年影响因子：4.795 ）

ISSN： 0016-6731

年卷期： 2022 年 220 卷 4 期

页码：

收录情况： SCI

摘要： In plants, large numbers of R genes, which segregate as loci with alternative alleles conferring different levels of disease resistance to pathogens, have been maintained over a long period of evolution. The reason why hosts harbor susceptible alleles in view of their null contribution to resistance is unclear. In rice, a single copy gene, Pi-ta, segregates for 2 expressed clades of alleles, 1 resistant and the other susceptible. We simulated loss-of-function of the Pi-ta susceptible allele using the CRISPR/Cas9 system to detect subsequent fitness changes and obtained insights into fitness effects related to the retention of the Pi-ta susceptible allele. Our creation of an artificial knockout of the Pi-ta susceptible allele suffered fitness-related trait declines of up to 49% in terms of filled grain yield upon the loss of Pi-ta function. The Pi-ta susceptible alleles might serve as an off-switch to downstream immune signaling, thus contributing to the fine-tuning of plant defense responses. The results demonstrated that the susceptible Pi-ta alleles should have evolved pleiotropic functions, facilitating their retention in populations. As Pi-ta is a single copy gene with no paralogs in the genome, its function cannot be compensated by an alternative gene; whereas most other R genes form gene clusters by tandem duplications, and the function could be compensated by paralogs with high sequence similarity. This attempt to evaluate the fitness effects of the R gene in crops indicates that not all disease resistance genes incur fitness costs, which also provides a plausible explanation for how host genomes can tolerate the possible genetic load associated with a vast repertoire of R genes.

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