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DNA Methylation Alterations and Their Association with High Temperature Tolerance in Rice Anthesis

文献类型： 外文期刊

作者： Li, Bo 1 ; Cai, Haiya 2 ; Liu, Ke 1 ; An, Bingzhuang 1 ; Wang, Rong 1 ; Yang, Fang 3 ; Zeng, Changli 4 ; Jiao, Chunhai 2 ; Xu, Yanhao 2 ;

作者机构： 1.Yangtze Univ, Hubei Key Lab Waterlogging Disaster & Agr Use Wet, Hubei Collaborat Innovat Ctr Grain Ind, Minist Educ,Engn Res Ctr Ecol & Agr Use Wetland, Jingzhou 434025, Hubei, Peoples R China

2.Hubei Acad Agr Sci, Food Crops Inst, Hubei Key Lab Food Crop Germplasm & Genet Improve, Wuhan 430064, Hubei, Peoples R China

3.Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan 430072, Hubei, Peoples R China

4.Jianghan Univ, Hubei Engn Res Ctr Protect & Utilizat Special Bio, Wuhan 430056, Hubei, Peoples R China

关键词： DNA methylation; High temperature tolerance; MSAP; Rice (Oryza sativa; L; )

期刊名称：JOURNAL OF PLANT GROWTH REGULATION （ 影响因子：4.64； 五年影响因子：4.469 ）

ISSN： 0721-7595

年卷期：

页码：

收录情况： SCI

摘要： DNA methylation is an important epigenetic mechanism involved in gene regulation under environmental stresses in plants. However, little information is available regarding its responses to high temperature (HT) and association with HT tolerance in rice. In this study, fourteen rice genotypes were classified into the susceptible, moderate, and tolerant groups by the high temperature susceptibility index (HTSI) after HT treatment. The changes of DNA methylation in rice anthesis under normal and HT30 conditions were investigated using methylation-sensitive amplified polymorphism31 (MSAP). The MSAP results showed that the DNA methylation level significantly increased in the susceptible rice group and decreased in the tolerant rice group under HT treatment, while no significant difference was observed in the moderate rice group. More hypomethylation events were detected in the tolerant rice group, while more hypermethylation was detected in the susceptible rice group. Forty-four differentially methylated epiloci (DME) were generated under both control and HT conditions, which can clearly distinguish the susceptible, moderate, and tolerant genotypes via PCoA analysis. Approximately 43.18% of DMEs were determined to be tolerance-associated epiloci (TAEs). 63.15% TAEs were sequenced and annotated into 12 genes. Quantitative RT-PCR analysis showed that 12 TAE genes were mainly upregulated in 14 rice genotypes, and their expression levels were related to the HT tolerance of rice. Here, DEGs, generated from a number of genotypes, indicate higher probabilities for association with stress tolerance. Overall, these results suggest that DNA methylation regulation might play a key role in adaptation to HT stress in rice.

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