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Identification of CaPs locus involving in purple stripe formation on unripe fruit, reveals allelic variation and alternative splicing of R2R3-MYB transcription factor in pepper (Capsicum annuum L.)

文献类型： 外文期刊

作者： Li, Ning 1 ; Liu, Yabo 1 ; Yin, Yanxu 1 ; Gao, Shenghua 1 ; Wu, Fangyuan 1 ; Yu, Chuying 1 ; Wang, Fei 1 ; Kang, Byoung-Cheorl 3 ; Xu, Kai 1 ; Jiao, Chunhai 1 ; Yao, Minghua 1 ;

作者机构： 1.Hubei Acad Agr Sci, Cash Crops Res Inst, Hubei Key Lab Vegetable Germplasm Innovat & Genet, Wuhan, Peoples R China

2.Yangtze Univ, Coll Hort & Gardening, Jingzhou, Peoples R China

3.Seoul Natl Univ, Plant Genom Breeding Inst, Res Inst Agr & Life Sci, Coll Agr & Life Sci,Dept Agr Forestry & Bioresourc, Seoul, South Korea

4.Hubei Hongshan Lab, Wuhan, Peoples R China

关键词： pepper; anthocyanin biosynthesis; fine mapping; purple stripe; transcriptional reputational

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： The purple color of unripe pepper fruit is attributed to the accumulation of anthocyanins. Only a few genes controlling the biosynthesis and regulation of anthocyanins have been cloned in Capsicum. In this study, we performed a bulked segregant analysis of the purple striped trait using an F-2 population derived from a cross between the immature purple striped fruit line Chen12-4-1-1-1-1 and the normal green fruit line Zhongxian101-M-F-9. We mapped the CaPs locus to an 841.39 kb region between markers M-CA690-Xba and MCA710-03 on chromosome 10. CA10g11690 encodes an R2R3-MYB transcription factor that is involved in the biosynthesis of anthocyanins as the best candidate gene. Overexpression and silencing in transformed tobacco (Nicotiana tabacum) lines indicated that CA10g11690 is involved in the formation of purple stripes in the exocarp. A comparison of parental sequences identified an insertion fragment of 1,926 bp in the second intron region of Chen12-4, and eight SNPs were detected between the two parents. Additionally, there were 49 single nucleotide polymorphic variations, two sequence deletions, and four sequence insertions in the promoter region. We found that CA10g11690 undergoes alternative splicing and generates different transcripts. Thus, the functional transcript of CA10g11690 appeared to be primarily involved in the development of purple phenotype in the exocarp. Our data provide new insight into the mechanism of anthocyanin biosynthesis and a theoretical basis for the future breeding of purple striped pepper varieties.

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