Ultrasensitive and rapid detection of organophosphates using a dual-signal naked-eye hydrogel sensor based on acetylcholinesterase inhibition
文献类型： 外文期刊
作者： Wang, Jiachun 1 ; Huang, Yibing 1 ; Kuai, Ziyu 1 ; Zhang, Yanping 1 ; Shen, Qi 1 ; Tian, Pujing 1 ; Nong, Weihai 1 ; Jiang, Wantong 1 ; He, Yuting 1 ; Ran, Nana 1 ; Yin, Yinuo 1 ; Li, Tiezhu 2 ; Luo, Quan 1 ;
作者机构： 1.Jilin Univ, Sch Life Sci, Key Lab Mol Enzymol & Engn, Minist Educ, Changchun 130012, Peoples R China
2.Jilin Acad Agr Sci, Inst Agrofood Technol, Changchun 130033, Peoples R China
3.Jilin Univ, Coll Chem, Ctr Supramol Chem Biol, State Key Lab Supramol Struct & Mat, Changchun 130012, Peoples R China
关键词： Acetylcholinesterase inhibition; Gold nanozyme; Organophosphorus pesticide; Dual signal readout; Self-calibration method
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）
ISSN： 0141-8130
年卷期： 2024 年 283 卷
页码：
收录情况： SCI
摘要： Accurate determination of pesticide residues is crucial for food safety. A self-calibration method was developed for dual-signal "naked-eye" detection of organophosphorus pesticides (OPs) using bifunctional gold nanozymes (AuNEs). OPs inhibit the cascade reaction of acetylcholinesterase/choline oxidase (AChE/CHO) to reduce hydrogen peroxide (H2O2) production, which affects the AuNE-catalyzed color reaction and quenches the fluorescence of AuNEs with the assistance of Fe2+. The multi-enzyme cascade system can be integrated into hydrogel sheets for smartphone-assisted digital, quantitative, and onsite detection of OPs in real food samples by analyzing the linear changes in colorimetric and fluorometric signals, which closely match liquid chromatography-mass spectrometry (LC-MS) results. This method displays a lowest 'naked-eye' detection concentration of 7 ppb, a wide linear range (0-2000 ng/mL), and particularly the advantage of anti-interference via self-calibration when one of the signals is abnormal.
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