Functional Differentiation of the Succinate Dehydrogenase Subunit SdhC Governs the Sensitivity to SDHI Fungicides, ROS Homeostasis, and Pathogenicity in Fusarium asiaticum
文献类型： 外文期刊
作者： Chen, Wenchan 1 ; Li, Xiujuan 1 ; Wei, Lingling 1 ; Chen, Bin 1 ; Han, Chenyang 3 ; Duan, Yabing 1 ; Chen, Changjun 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China
3.Nanjing Agr Univ, Coll Hort, Ctr Pear Engn Technol Res, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China
关键词： F. asiaticum; SdhC subunits; virulence; ROS homeostasis; SDHI sensitivity
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）
ISSN： 0021-8561
年卷期： 2024 年 72 卷 18 期
页码：
收录情况： SCI
摘要： Succinate dehydrogenase (SDH) is an integral component of the tricarboxylic acid cycle (TCA) and respiratory electron transport chain (ETC), targeted by succinate dehydrogenase inhibitors (SDHIs). Fusarium asiaticum is a prominent phytopathogen causing Fusarium head blight (FHB) on wheat. Here, we characterized the functions of the FaSdhA, FaSdhB, FaSdhC(1), FaSdhC(2), and FaSdhD subunits. Deletion of FaSdhA, FaSdhB, or FaSdhD resulted in significant growth defects in F. asiaticum. The FaSdhC(1) or FaSdhC(2) deletion mutants exhibited substantial reductions in fungal growth, conidiation, virulence, and reactive oxygen species (ROS). The FaSdhC(1) expression was significantly induced by pydiflumetofen (PYD). The Delta FaSdhC(1) mutant displayed hypersensitivity to SDHIs, whereas the Delta FaSdhC(2) mutant exhibited resistance against most SDHIs. The transmembrane domains of FaSdhC(1) are essential for regulating mycelial growth, virulence, and sensitivity to SDHIs. These findings provided valuable insights into how the two SdhC paralogues regulated the functional integrity of SDH, ROS homeostasis, and the sensitivity to SDHIs in phytopathogenic fungi.
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