Functional Plasticity, Redundancy, and Specificity of Lanosterol 14α-Demethylase in Regulating the Sensitivity to DMIs in Calonectria ilicicola
文献类型： 外文期刊
作者： Wei, Lingling 1 ; Shi, Haiping 1 ; Chen, Bin 1 ; Li, Xiujuan 1 ; Chen, Wenchan 2 ; Wu, Chengdong 3 ; Gai, Yunpeng 4 ; Chen, Changjun 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210095, Jiangsu, Peoples R China
3.Pukou Dist Modern Agr Dev Serv Ctr Nanjing City, Nanjing 211800, Jiangsu, Peoples R China
4.Beijing Forestry Univ, Sch Grassland Sci, Beijing 100083, Peoples R China
关键词： C. ilicicola; sterol 14 alpha-demethylase; novel genus-specific CYP51 function; DMI sensitivity; molecular docking
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 2022影响因子：6.1； 五年影响因子：6.3 ）
ISSN： 0021-8561
年卷期： 2024 年 72 卷 15 期
收录情况： SCI
摘要： Cytochrome P450 sterol 14 alpha-demethylase (CYP51) is a key enzyme involved in the sterol biosynthesis pathway and serves as a target for sterol demethylation inhibitors (DMIs). In this study, the 3D structures of three CPY51 paralogues from Calonectria ilicicola (C. ilicicola) were first modeled by AlphaFold2, and molecular docking results showed that CiCYP51A, CiCYP51B, or CiCYP51C proteins individually possessed two active pockets that interacted with DMIs. Our results showed that the three paralogues play important roles in development, pathogenicity, and sensitivity to DMI fungicides. Specifically, CiCYP51A primarily contributed to cell wall integrity maintenance and tolerance to abiotic stresses, and CiCYP51B was implicated in sexual reproduction and virulence, while CiCYP51C exerted negative regulatory effects on sterol 14 alpha-demethylase activity within the ergosterol biosynthetic pathway, revealing its genus-specific function in C. ilicicola. These findings provide valuable insights into developing rational strategies for controlling soybean red crown rot caused by C. ilicicola.
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