The natural polycyclic tetramate macrolactam HSAF inhibit Fusarium graminearum through altering cell membrane integrity by targeting FgORP1
文献类型： 外文期刊
作者： Chen, Wenchan 1 ; Tang, Bao 1 ; Hou, Rongxian 1 ; Sun, Weibo 1 ; Han, Chenyang 3 ; Guo, Baodian 1 ; Zhao, Yangyang 1 ; Li, Chaohui 1 ; Sheng, Cong 1 ; Zhao, Yancun 1 ; Liu, Fengquan 1 ;
作者机构： 1.Inst Plant Protect, Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base Minist Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China
2.Guizhou Univ, Coll Agr, Dept Plant Pathol, Key Lab Agr Microbiol, Guiyang 550025, Guizhou, Peoples R China
3.Nanjing Agr Univ, Coll Hort, Ctr Pear Engn Technol Res, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China
关键词： F. graminearum; HSAF; FgORP1; Ergosterol; Cell membrane
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）
ISSN： 0141-8130
年卷期： 2024 年 261 卷
页码：
收录情况： SCI
摘要： Fusarium graminearum is a dominant phytopathogenic fungus causing Fusarium head blight (FHB) in cereal crops. Heat-stable antifungal factor (HSAF) is a polycyclic tetramate macrolactam (PoTeM) isolated from Lysobacter enzymogenes that exhibits strong antifungal activity against F. graminearum. HSAF significantly reduces the DON production and virulence of F. graminearum. Importantly, HSAF exhibited no cross-resistance to carbendazim, phenamacril, tebuconazole and pydiflumetofen. However, the target protein of HSAF in F. graminearum is unclear. In this study, the oxysterol-binding protein FgORP1 was identified as the potential target of HSAF using surface plasmon resonance (SPR) combined with RNA-sequence (RNA-seq). The RNA-seq results showed cell membrane and ergosterol biosynthesis were significantly impacted by HSAF in F. graminearum. Molecular docking showed that HSAF binds with arginine 1205 and glutamic acid 1212, which are located in the oxysterolbinding domain of FgORP1. The two amino acids in FgORP1 are responsible for HSAF resistance in F. graminearum though site-directed mutagenesis. Furthermore, deletion of FgORP1 led to significantly decreased sensitivity to HSAF. Additionally, FgORP1 regulates the mycelial growth, conidiation, DON production, ergosterol biosynthesis and virulence in F. graminearum. Overall, our findings revealed the mode of action of HSAF against F. graminearum, indicating that HSAF is a promising fungicide for controlling FHB.
- 相关文献
作者其他论文 更多>>
-
OsPRMT5 methylates OsPAL1 to promote rice resistance, hindered by a Xanthomonas oryzae effector
作者：Sheng, Cong;Wang, Bo;Chen, Wenchan;Guo, Baodian;Zhao, Yancun;Liu, Fengquan;Li, Kaihuai;Liu, Fengquan;Wang, Bo;Chen, Wenchan;Qiao, Lulu;Zhao, Hongwei
关键词：effector; OsPAL1; OsPRMT5; protein arginine methylation; rice bacterial blight; SA
-
Sigma factor 70 RpoD contributes to virulence by regulating cell motility, oxidative stress tolerance, and manipulating the expression of hrpG and hrpX in Xanthomonas oryzae pv. oryzae
作者：Xu, Zhizhou;Liu, Fengquan;Liu, Fengquan;Xu, Zhizhou;Wang, Bo;Guo, Baodian;Sheng, Cong;Zhao, Yangyang;Tang, Bao;Zhao, Yancun;Liu, Fengquan;Wu, Guichun
关键词：Oryza sativa; sigma factor; pathogenicity; transcriptional regulation; type III secretion system
-
Apoplastic proteomic reveals Colletotrichum fructicola effector CfXyn11A recognized by tobacco and suppressed by pear in the apoplast
作者：Han, Chenyang;Tao, Shutian;Xie, Zhihua;Zhang, Shaoling;Liu, Fengquan;Liu, Fengquan
关键词：
Colletotrichum fructicola ; Pear; Apoplastic plant-microbe interaction; Fungal effector; CfXyn11A -
Lysobacter enzymogenes: A fully armed biocontrol warrior
作者：Lin, Long;Shao, Xiaolong;Yang, Yicheng;Murero, Aprodisia Kavutu;Wang, Limin;Han, Sen;Su, Zhenhe;Qian, Guoliang;Xu, Gaoge;Zhao, Yangyang;Xu, Kangwen;Liu, Fengquan;Han, Sen;Xu, Kangwen;Yang, Mingming;Liao, Jinxing;Li, Kaihuai;Liu, Fengquan
关键词：Lysobacter enzymogenes; antimicrobial metabolites; loss of flagella; type IV secretion system
-
Kasugamycin disrupts membrane transport and reduces virulence in Pectobacterium aroidearum to control konjac soft rot
作者：Xiong, Chunlan;Zhou, Xue;Zhu, Yu;Du, Xiaojuan;Li, Kaihuai;Liu, Fengquan;Xiong, Chunlan;Zhou, Xue;Zhu, Yu;Du, Xiaojuan;Li, Kaihuai;Liu, Fengquan;Tu, Yupei;Li, Kaihuai;Liu, Fengquan
关键词：Konjac; Soft rot;
Pectobacterium aroidearum ; Kasugamycin; Antibacterial activity -
First Report of Bacterial Fruit Blotch Caused by Kosakonia cowanii on Trichosanthis fructus in Jiangsu Province, China
作者：Chen, Xian;Tang, Bao;Zhao, Yancun;Liu, Fengquan;Chen, Xian;Tang, Bao;Laborda, Pedro
关键词：bacterial fruit blotch; Kosakonia cowanii; Trichosanthis fructus
-
The HOG-pathway related AaOS1 leads to dicarboximide-resistance in field strains of Alternaria alternata and contributes, together with the Aafhk1, to mycotoxin production and virulence
作者：Chen, Changjun;Wang, Xiaoyu;Wei, Lingling;Chen, Bin;Li, Xiujuan;Shi, Haiping;Xie, Shuai;Hu, Hao;Chen, Changjun;Chen, Wenchan;Wei, Lihui;Wang, Xiaoyu
关键词：garlic leaf spot; Alternaria alternata; dicarboximides; mutations; mycotoxin AOH
