Identification and Analysis of Polychlorinated Biphenyls (PCBs)-Biodegrading Bacterial Strains in Shanghai
文献类型： 外文期刊
作者： Shuai, Jian-Jun 1 ; Tian, Yong-Sheng 2 ; Yao, Quan-Hong 2 ; Peng, Ri-He 2 ; Xiong, Fei 1 ; Xiong, Ai-Sheng 2 ;
作者机构： 1.Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou 225009, Peoples R China
2.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai, Peoples R China
关键词： 16S ribosomal DNA;polychlorinated biphenyls: pollutant;environmental contaminant genome;industrial contamination;agricultural contamination
期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.188； 五年影响因子：2.197 ）
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年卷期：
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收录情况： SCI
摘要： As one of China's great metropolises, Shanghai is vulnerable to various forms of industrial and agricultural contamination associated with its development. Polychlorinated biphenyls (PCBs) are man-made chemicals that never existed in nature until the 1900s when they started to be released into the environment. PCBs are hazardous environmental contaminants that bind strongly to soil. In this study, four soil samples were screened for the presence of PCB-degrading bacteria. The 16 S rDNAs were amplified from those genomes and the products (similar to 1.5 kb) were purified and sequenced for the isolation and identification of bacterial species. Four Pseudomonas strains (strain 1-212 from sample 1; strain 2-241 from sample 2; strain 3-318 from sample 3; and strain 4-150 from sample 4) were selected for analysis by HPLC. Setting the content of the biphenyl in CK as 100%, the biphenyl contents was 2.32% in 1-212, 73.11% in 2-241, 69.83% in 3-318, and 86.16% in 4-150. The results of this study suggest directions for future research, including genetic screening, cloning and restructuring, and provide guidance for the cultivation of PCBs-degrading bacteria.
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