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Effect of VP28 DNA vaccine on white spot syndrome virus in Litopenaeus vannamei

文献类型： 外文期刊

作者： Li, Xiang 1 ; Liu, Qing-hui 1 ; Hou, Lin 2 ; Huang, Jie 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Liaoning Normal Univ, Dept Biol, Dalian 116029, Liaoning, Peoples R China

关键词： VP28 DNA vaccine: immunologic-drug;immunostimulant-drug;vaccine

期刊名称：AQUACULTURE INTERNATIONAL （ 影响因子：2.235； 五年影响因子：2.616 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： White spot syndrome virus (WSSV) occurs worldwide and causes high mortality and considerable economic damage to the shrimp farming industry. Considering the global environmental, the economic and sociological importance of shrimp farming, and the constraints of high intensity cultivation, development of novel control measures against the outbreak of WSSV become inevitable. In this study, we have explored the protective efficacy of DNA vaccination and tissue distribution of the recombinant plasmid in immunized Litopenaeus vannamei. The VP28 gene was cloned in the eukaryotic expression vector pVAX1, and the construct vector was named as lpv28. The protective effect of lpv28 against WSSV was evaluated in L. vannamei by injecting lpv28 construct and later challenging with WSSV. Expression of these proteins from the recombinant plasmids was confirmed in vitro by RT-PCR and Western blot analysis. The result of vaccination trials showed that a survival rate in shrimp vaccinated with lpv28 was 52.5% at most compared to control groups (100% mortality). The immunological parameters analyzed in the vaccinated and control groups showed that the vaccinated groups owned a high level of lysozyme, alkaline phosphatase, and total superoxide dismutase when compared to the control group. Furthermore, protein expression analysis indicated that VP28 can be detected in gill, muscle and head soft tissue of the shrimps in the immunized group after 14th day injection. Thus, the result indicated that DNA vaccination strategy has a potential utility against WSSV.

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