您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Effect of VP28 DNA vaccine on white spot syndrome virus in Litopenaeus vannamei

文献类型： 外文期刊

作者： Li, Xiang 1 ; Liu, Qing-hui 1 ; Hou, Lin 2 ; Huang, Jie 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Liaoning Normal Univ, Dept Biol, Dalian 116029, Liaoning, Peoples R China

关键词： VP28 DNA vaccine: immunologic-drug;immunostimulant-drug;vaccine

期刊名称：AQUACULTURE INTERNATIONAL （ 影响因子：2.235； 五年影响因子：2.616 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： White spot syndrome virus (WSSV) occurs worldwide and causes high mortality and considerable economic damage to the shrimp farming industry. Considering the global environmental, the economic and sociological importance of shrimp farming, and the constraints of high intensity cultivation, development of novel control measures against the outbreak of WSSV become inevitable. In this study, we have explored the protective efficacy of DNA vaccination and tissue distribution of the recombinant plasmid in immunized Litopenaeus vannamei. The VP28 gene was cloned in the eukaryotic expression vector pVAX1, and the construct vector was named as lpv28. The protective effect of lpv28 against WSSV was evaluated in L. vannamei by injecting lpv28 construct and later challenging with WSSV. Expression of these proteins from the recombinant plasmids was confirmed in vitro by RT-PCR and Western blot analysis. The result of vaccination trials showed that a survival rate in shrimp vaccinated with lpv28 was 52.5% at most compared to control groups (100% mortality). The immunological parameters analyzed in the vaccinated and control groups showed that the vaccinated groups owned a high level of lysozyme, alkaline phosphatase, and total superoxide dismutase when compared to the control group. Furthermore, protein expression analysis indicated that VP28 can be detected in gill, muscle and head soft tissue of the shrimps in the immunized group after 14th day injection. Thus, the result indicated that DNA vaccination strategy has a potential utility against WSSV.

  • 相关文献

[1]A multivalent killed whole-cell vaccine induces effective protection against Edwardsiella tarda and Vibrio anguillarum. Sun, Yun,Sun, Li,Liu, Chun-sheng,Sun, Yun.

[2]Comparative study of the immune effect of an Edwardsiella tarda antigen in two forms: Subunit vaccine vs DNA vaccine. Sun, Yun,Sun, Li,Sun, Yun,Liu, Chun-Sheng.

[3]Protein encoded by ORF093 is an effective vaccine candidate for infectious spleen and kidney necrosis virus in Chinese perch Siniperca chuatsi. Li, Ningqiu,Fu, Xiaozhe,Guo, Huizhi,Lin, Qiang,Liu, Lihui,Zhang, Defeng,Fang, Xiang,Wu, Shuqin,Li, Ningqiu,Fu, Xiaozhe,Guo, Huizhi,Lin, Qiang,Liu, Lihui,Zhang, Defeng,Fang, Xiang,Wu, Shuqin.

[4]Early protein ORF086 is an effective vaccine candidate for infectious spleen and kidney necrosis virus in mandarin fish Siniperca chuatsi. Fu, Xiaozhe,Li, Ningqiu,Lin, Qiang,Guo, Huizhi,Liu, Lihui,Huang, Zhibin,Wu, Shuqin,Fu, Xiaozhe,Li, Ningqiu,Lin, Qiang,Guo, Huizhi,Liu, Lihui,Wu, Shuqin.

[5]Development and efficacy of a novel streptomycin-resistant Flavobacterium johnsoniae vaccine in grass carp (Ctenopharyngodon idella). Li, Ningqiu,Lin, Qiang,Fu, Xiaozhe,Guo, Huizhi,Liu, Lihui,Wu, Shuqin,Li, Ningqiu,Li, Ningqiu,Lin, Qiang,Fu, Xiaozhe,Guo, Huizhi,Liu, Lihui,Wu, Shuqin.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台