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Optimizing the binding activity of the AP2/ERF transcription factor with the GCC box element from Brassica napus by directed evolution

文献类型： 外文期刊

作者： Jin, Xiao-Fen 1 ; Zhu, Bo 1 ; Peng, Ri-He 1 ; Jiang, Hai-hua 1 ; Chen, Jian-Min 2 ; Zhuang, Jing 3 ; Zhang, Jian 3 ; Yao, 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China

2.Yangzhou Univ, Coll Life Sci & Technol, Jiangsu, Peoples R China

3.Alberta Res Council, Vegreville, AB T9C 1T4, Canada

关键词： AP2/ERF;GCC box;Gene shuffling;Transcription factor;Yeast one-hybrid system

期刊名称：BMB REPORTS （ 影响因子：4.778； 五年影响因子：4.377 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： In this study, we cloned the ERF-B3 subfamily transcription factor gene BnaERF-B3-hy15 from Brassica napus L. Huyou15. This 600 bp gene encodes a 199 amino acid classic ethylene responsive factor (ERF), which shown no binding or very weak binding GCC box-binding activity by the yeast one-hybrid assay. We used gene shuffling and the yeast one-hybrid system to obtain three mutated sequences that can bind to the GCC box. Sequence analysis indicated that two residues, Gly156 in the AP2 domain and Phe62 at the N-terminal domain were mutated to arginine and serine, respectively. Changes of Gly156 to arginine and Phe62 to serine increased the GCC-binding activity of BnaERF-B3-hy15 and the alter of Gly156 to arginine changed the AP2-domain structure of BnaERF-B3-hy15. [BMB reports 2010; 43(8): 567-572]

  • 相关文献

[1]Discovery and expression profile analysis of AP2/ERF family genes from Triticum aestivum. Zhuang, Jing,Yao, Quan-Hong,Sun, Chao-Cai,Zhou, Xi-Rong,Xiong, Ai-Sheng,Zhuang, Jing,Zhang, Jian,Chen, Jian-Min,Xiong, Fei.

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[4]Expression and Function of a Modified AP2/ERF Transcription Factor from Brassica napus Enhances Cold Tolerance in Transgenic Arabidopsis. Xiong, Ai-Sheng,Wang, Feng,Jiang, Hai-Hua,Peng, Ri-He,Jin, Xiao-Fen,Zhu, Bo,Yao, Quan-Hong,Zhuang, Jing,Zhang, Jian.

[5]Analysis of Brassica rapa ESTs: gene discovery and expression patterns of AP2/ERF family genes. Zhuang, Jing,Xiong, Ai-Sheng,Peng, Ri-He,Gao, Feng,Zhu, Bo,Fu, Xiao-Yan,Jin, Xiao-Feng,Yao, Quan-Hong,Zhang, Zhen,Qiao, Yu-Shan,Zhuang, Jing,Zhang, Jian,Chen, Jian-Min.

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[11]Tomato stress-responsive factor TSRF1 interacts with ethylene responsive element GCC box and regulates pathogen resistance to Ralstonia solanacearum. Zhang, HB,Zhang, DB,Chen, J,Yang, YD,Huang, ZJ,Huang, DF,Wang, XC,Huang, RF. 2004

[12]A Rice OsAP23, Functioning as an AP2/ERF Transcription Factor, Reduces Salt Tolerance in Transgenic Arabidopsis. Zhuang, Jing,Wang, Feng,Xiong, Ai-Sheng,Jiang, Hai-Hua,Peng, Ri-He,Yao, Quan-Hong.

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[14]Isolation and characterization of a novel cDNA encoding ERF/AP2-type transcription factor OsAP25 from Oryza sativa L.. Fu, Xiao-Yan,Zhang, Zhen,Peng, Ri-He,Xiong, Ai-Sheng,Liu, Jin-Ge,Wu, Li-Juan,Gao, Feng,Zhu, Hong,Guo, Zhao-Kui,Yao, Quan-Hong. 2007

[15]Isolated and characterization of a cDNA encoding ethylene-responsive element binding protein (EREBP)/AP2-type protein, RCBF2, in Oryza sativa L.. Liu, Jin-Ge,Zhang, Zhen,Qin, Qiu-Lin,Peng, Ri-He,Xiong, Ai-Sheng,Chen, Jian-Min,Xu, Fang,Zhu, Hong,Yao, Quan-Hong.

[16]Isolation and characterization of an AP2/ERF-RAV transcription factor BnaRAV-1-HY15 in Brassica napus L. HuYou15. Zhuang, Jing,Sun, Chao-Cai,Zhou, Xi-Rong,Zhuang, Jing,Zhang, Jian,Xiong, Ai-Sheng. 2011

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