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Effect of trichostatin A on transfected donor cells and subsequent development of porcine cloned embryos

文献类型： 外文期刊

作者： Fu Bo 2 ; Liu Di 1 ; Fang Qing-chang 2 ; Ren Liang 2 ; Ma Hong 1 ; Wang Liang 1 ; Guo Zhen-hua 1 ; Li Zhong-qiu 1 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Inst Anim Sci, Harbin 150086, Peoples R China

2.NE Agr Univ, Coll Life Sci, Harbin 150030, Peoples R China

关键词： EGFP;Histone deacetylation;Porcine;Somatic cell nuclear transfer;Trichostatin A

期刊名称：ZYGOTE （ 影响因子：1.442； 五年影响因子：1.536 ）

ISSN：

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收录情况： SCI

摘要： Transgenes integrated into mammalian cells are silenced rapidly. This phenomenon correlates with repressed chromatin structure marked by histone hypoacetylation. This study investigated the effect of trichostatin A (TSA; a histone-deacetylase inhibitor) on EGFP expression in transfected cells and embryonic development after somatic cell nuclear transfer (SCNT). Porcine adult fibroblasts were transfected with a pEGFP-C1 vector. Then transfected cells, donor cells for SCNT, were pretreated with TSA, with the untreated cells being used as the control. Expression of EGFP in donor cells and reconstructed embryos was detected when exposed to blue light. Results showed that the percentage of EGFP-positive cells significantly increased when the transfected cells were treated with TSA and the increased expression of EGFP was sustained to at least the morula stage. In addition, the cytotoxic effect of TSA on the transfected cells was dose dependent. In conclusion, TSA can rescue the silenced EGFP gene. Even after transferring the TSA-treated cells to enucleated recipient oocytes, TSA retained the ability to rescue a silenced EGFP gene. In addition, TSA had an impact on cell proliferation.

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[1]Effect of Pyruvate and Lactic Acid on the Development of Porcine Embryos. Fu Bo,Llu Di,Ma Hong,Li Zhong-qiu,Wang Liang,Bai Jing. 2012

[2]Effect of Cycloheximide on the Development of Parthenogenetic Activation and Cloned Porcine Embryos. Ma, Hong,Fu, Bo,Li, Zhongqiu,Liu, Di,Zhao, Jinfeng,Fang, Qingchang,Ren, Liang. 2011

[3]Development of porcine tetraploid somatic cell nuclear transfer embryos is influenced by oocyte nuclei. Fu, Bo,Liu, Di,Ma, Hong,Guo, Zhen-Hua,Wang, Liang,Li, Zhong-Qiu,Peng, Fu-Gang,Liu, Di,Bai, Jing.

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[6]Functional characterization of genetic variants in the porcine TLR3 gene. Wang, L.,Chen, Y. C.,Li, H. T.,Yang, X. Q.,Wang, L.,Zhang, D. J.,Liu, D.. 2014

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[8]Characterization and functional significance of polymorphisms in porcine Toll-like receptor (TLR) 5 gene. Li, Hai-tao,Yang, Xiu-qin,Wang, Liang,Liu, Di. 2012

[9]Identification and functional analysis of a novel single nucleotide polymorphism (SNP) in the porcine Toll like receptor (TLR) 5 gene. Li, H. -T.,Yang, X. -Q.,Liu, D.. 2011

[10]Myeloid zinc finger 1 (MZF1) is the most important transcriptional factor for porcine follistatin promoter. Sun Ya-meng,Yang Xiu-qin,Liu Di,Wang Liang,Zhang Dong-jie,Liu Di. 2015

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