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Myeloid zinc finger 1 (MZF1) is the most important transcriptional factor for porcine follistatin promoter

文献类型： 外文期刊

作者： Sun Ya-meng 1 ; Wang Liang 2 ; Yang Xiu-qin 1 ; Zhang Dong-jie 2 ; Liu Di 1 ;

作者机构： 1.Northeast Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Peoples R China

2.Heilongjiang Acad Agr Sci, Harbin 150086, Peoples R China

关键词： promoter;follistatin;porcine;firefly luciferase;myeloid zinc finger 1

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2015 年 14 卷 7 期

页码：

收录情况： SCI

摘要： Follistatin (FS) is a secreted protein, which was originally isolated from porcine follicular fluid. Expression of follistatin is tightly regulated during porcine growth and development. To study the essential transcriptional regions of the porcine FS promoter, ten primer pairs were designed to amplify segments with different lengths of the FS promoter from 1800 to +16 bp. The products were then inserted into the pGL3-basic vector to analyze the relative luciferase activity. The results showed that the most remarkable changes of promoter activity were observed between constructs (-302/+16 bp)-FS and (-180/+16 bp)-FS (P<0.01). Further research showed that the reconstructed reporter plasmid lacking myeloid zinc finger 1 (MZF1) binding sequence had significantly decreased luciferase activity (P<0.05). Furthermore, the FS protein expression was significantly increased in PK15 cells while the MZF1 was overexpressed, suggesting that the short sequence "TCCCCACC" (the recognition site of transcription factor MZF1) was the most important for FS transcription activation in the porcine.

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