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Production of a monoclonal antibody by simultaneous immunization of staphylococcal enterotoxin A and B

文献类型： 外文期刊

作者： Liang, Bin 3 ; Zhang, Yongxia 3 ; Liu, Aiping 1 ; Zhou, Youxiang 4 ; Chen, Fusheng 1 ; Wang, Xiaohong 1 ;

作者机构： 1.Minist Agr, Key Lab Food Safety Evaluat, Wuhan 430070, Hubei Province, Peoples R China

2.Huazhong Agr Univ, Natl Key Lab Agromicrobiol China, Wuhan 430070, Hubei Province, Peoples R China

3.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei Province, Peoples R China

4.Agr Minist China, Food Qual Inspect & Testing Ctr, Wuhan 430064, Hubei Province, Peoples R China

关键词： Indirect competitive ELISA;Monoclonal antibody;Simultaneous immunization;Staphylococcal enterotoxin

期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：2.926； 五年影响因子：2.685 ）

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收录情况： SCI

摘要： In this paper, a method of simultaneous immunizing BALB/c mice with staphylococcal enterotoxin (SE) A and B (SEA and SEB) to prepare a monoclonal antibody (3F2) for detecting both of SEA and SEB was developed. The results showed that antibody 3F2 had high titers against both SEA and SEB by enzyme-linked immunosorbent assay (ELISA). The sensitivities of 3F2 to SEA and SEB detected by ELISA were 133.2 and 82.5 ng/mL, respectively, and the detection limits for the two enterotoxins were about 1 ng/mL. The antibody 3F2 had high specificities and affinities to both SEA and SEB, and had no cross-reaction with SEC1, bovine serum albumin, and ovalbumin. SEs-free skimmed milk samples were spiked with different concentrations of SEA, SEB, or both of them, respectively. Average recoveries of SEA and SEB from the spiked samples were all nearly between 82% and 104%. The result suggested that one cell fusion with simultaneous immunization by multiple antigen to prepare monoclonal antibody against them was possible, simple, and economic. The monoclonal antibody could be used in simultaneous detecting multifarious SEs.

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