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Hybrid Rubisco of tomato large subunits and tobacco small subunits is functional in tobacco plants

文献类型： 外文期刊

作者： Zhang, Xing-Hai 1 ; Webb, James 1 ; Huang, Yi-Hong 1 ; Lin, Li 1 ; Tang, Ri-Sheng 2 ; Liu, Aimin 3 ;

作者机构： 1.Florida Atlantic Univ, Dept Biol Sci, Boca Raton, FL 33431 USA

2.Jiangsu Acad Agr Sci, Inst Agrobiol Genet & Physiol, Nanjing 210014, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Biotechnol, Nanjing 210014, Peoples R China

关键词： carbon dioxide: 124-38-9;chlorophyll;carboxylase: 9031-55-4;ribulose-1;5-bisphosphate carboxylase-oxygenase: Rubisco;EC 4.1.1.39;cytosol-synthesized small subunits;chloroplast-made large subunits;enzyme activity;light condition;photoautotrophic growth;reproductive maturity;plant phenotype;early stage development;lower photosynthesis rate;plants reproduction

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

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收录情况： SCI

摘要： Biogenesis of functional ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) in plants requires specific assembly in the chloroplast of the imported, cytosol-synthesized small subunits (SS) with the chloroplast-made large subunits (LS). Accumulating evidence indicates that chloroplasts (plastids) generally have a low tolerance for assembling foreign or modified Rubisco. To explore Rubisco engineering, we created two lines of transplastomic tobacco plants whose rbcL gene was replaced by tomato-derived rbcL: plant LLS2 with Rubisco composed of tobacco SS and Q437R LS and plant LLS4 with a hybrid Rubisco of tobacco SS and tomato LS (representing four substitutions of Y226F, A230T, S279T and Q437R from tobacco LS). Plant LLS2 exhibited similar phenotypes as the wild type. Plant LLS4 showed lower chlorophyll and Rubisco levels particularly in young emerging leaves, lower photosynthesis rates and biomass during early stages of development, but was able to reach reproductive maturity and somewhat wild type-like phenotype under ambient CO2 condition. In vitro assays detected similar carboxylase activity and RuBP affinity in LLS2 and LLS4 plants as in wild type. Our studies demonstrated that tomato LS was sufficiently assembled with tobacco SS into functional Rubisco. The hybrid Rubisco of tomato LS and tobacco SS can drive photosynthesis that supports photoautotrophic growth and reproduction of tobacco plants under ambient CO2 and light conditions. We discuss the effect of these residue substitutions on Rubisco activity and the possible attribution of chlorophyll deficiency to the in planta photosynthesis performance in the hybrid Rubisco plants

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