文献类型： 外文期刊

作者： Zhang, Xing-Hai ¹ ; Keating, Patricia ¹ ; Wang, Xia-Wei ² ; Huang, Yi-Hong ¹ ; Martin, James ¹ ; Hartmann, James X. ¹ ;

作者机构： 1.Florida Atlantic Univ, Dept Biol Sci, Boca Raton, FL 33431 USA

2.Shandong Univ, Prov Hosp, Jinan 250021, Shandong, Peoples R China

3.Jiangsu Acad Agr Sci, Nanjing 210014, Jiangsu, Peoples R China

关键词： Chloroplast;Gene containment;Genetic engineering;Human interleukin-2 (hIL-2);Tobacco (Nicotiana tabacum)

期刊名称：MOLECULAR BIOTECHNOLOGY （ 影响因子：2.695； 五年影响因子：2.303 ）

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收录情况： SCI

摘要： Interleukin-2 (IL-2) is an important T lymphocyte- derived cytokine in the mammalian immune system. Non-native, recombinant IL-2 derived from Escherichia coli is used widely in both medical research and treatment of diseases. Recombinant human IL-2 gene has been expressed in plant nuclear genomes, therefore it can be spread to the environment through pollen. Furthermore, all the plant-produced IL-2 reported thus far had been attached with artificial tags or fusion proteins, which may trigger unintended immunological responses and therefore compromise its full utility as a medicine. To expand the potential of using plant chloroplasts to produce functional native human therapeutic proteins, we inserted an engineered human interleukin-2 (hIL-2)-coding gene, without any tags, into the chloroplast genome of tobacco (Nicotiana tabacum L.). Partially purified hIL-2 protein from the leaves of the transplastomic plants induced in vitro proliferation of IL-2-dependent murine T lymphocytes. Our study demonstrates that plant chloroplasts can serve as a bio-factory for production of an active native human interleukin in a self-contained and therefore environmentally safe manner.