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Phylogenetic and expression analysis of the angiopoietin-like gene family and their role in lipid metabolism in pigs

文献类型： 外文期刊

作者： Zheng, Zibin 1 ; Lyu, Wentao 1 ; Hong, Qihua 3 ; Yang, Hua 1 ; Li, Ying 4 ; Zhao, Shengjun 5 ; Ren, Ying 5 ; Xiao, Yingping 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

2.China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100193, Peoples R China

3.Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Peoples R China

4.Foshan Univ, Coll Life Sci & Engn, Guangdong Prov Key Lab Anim Mol Design & Precise B, Foshan 528000, Peoples R China

5.Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China

关键词： Angiopoietin-like Protein; Evolution; Expression; Fat Metabolize; Jinhua Pig

期刊名称：ANIMAL BIOSCIENCE （ 影响因子：2.2； 五年影响因子：2.3 ）

ISSN： 2765-0189

年卷期： 2023 年 36 卷 10 期

页码：

收录情况： SCI

摘要： Objective: The objective of this study was to investigate the phylogenetic and expression analysis of the angiopoietin-like (ANGPTL) gene family and their role in lipid metabolism in pigs. Methods: In this study, the amino acid sequence analysis, phylogenetic analysis, and chromosome adjacent gene analysis were performed to identify the ANGPTL gene family in pigs. According to the body weight data from 60 Jinhua pigs, different tissues of 6 pigs with average body weight were used to determine the expression profile of ANGPTL1-8. The ileum, subcutaneous fat, and liver of 8 pigs with distinct fatness were selected to analyze the gene expression of ANGPTL3, ANGPTL4, and ANGPTL8. Results: The sequence length of ANGPTLs in pigs was between 1,186 and 1,991 bp, and the pig ANGPTL family members shared common features with human homologous genes, including the high similarity of the amino acid sequence and chromosome flanking genes. Amino acid sequence analysis showed that ANGPTL1-7 had a highly conserved domain except for ANGPTL8. Phylogenetic analysis showed that each ANGPTL homologous gene shared a common origin. Quantitative reverse-transcription polymerase chain reaction analysis showed that ANGPTL family members had different expression patterns in different tissues. ANGPTL3 and ANGPTL8 were mainly expressed in the liver, while ANGPTL4 was expressed in many other tissues, such as the intestine and subcutaneous fat. The expression levels of ANGPTL3 in the liver and ANGPTL4 in the liver, intestine and subcutaneous fat of Jinhua pigs with low propensity for adipogenesis were significantly higher than those of high propensity for adipogenesis. Conclusion: These results increase our knowledge about the biological role of the ANGPTL family in this important economic species, it will also help to better understand the role of ANGPTL3, ANGPTL4, and ANGPTL8 in lipid metabolism of pigs, and provide innovative ideas for developing strategies to improve meat quality of pigs.

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