Essential role of the interaction between classical swine fever virus core protein and cellular MYO1B in viral components transport to exosomes and titer maintenance
文献类型： 外文期刊
作者： Bao, Xi 1 ; Zhuang, Tenhan 2 ; Xu, Yue 2 ; Chen, Li 2 ; Feng, Lei 2 ; Yao, Huochun 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Nanjing 210095, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Vet Immunol & Engn, Natl Res Ctr Engn & Technol Vet Biol, 50 Zhong ling St, Nanjing 210014, Jiangsu, Peoples R China
3.GuoTai Taizhou Ctr Technol Innovat Vet Biol, Taizhou 225300, Peoples R China
4.Minist Sci & Technol, Lab Food Qual & Safety, State Key Lab Cultivat Base, Nanjing, Jiangsu, Peoples R China
5.Jiangsu Univ, Sch Pharm, Zhenjiang, Jiangsu, Peoples R China
关键词： Classical Swine Fever Virus (CSFV); Core Protein; Myosin 1B; Exosomes
期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：2.7； 五年影响因子：2.9 ）
ISSN： 0378-1135
年卷期： 2024 年 299 卷
页码：
收录情况： SCI
摘要： Classical swine fever (CSF) is a severe disease caused by the highly contagious CSFV. Our previous study demonstrated that exosomes from CSFV-infected cells contained significant amounts of viral genome and Core (C) protein and were infectious. To further elucidate the mechanisms underlying the formation of these infectious exosomes, we investigated the intracellular transport of the C protein in this study. We first identified the synchronized transport of the C protein and viral genome to exosomes, distinguishing it from other structural proteins. This suggests that the C protein likely binds to the viral genome and is transported to exosomes as a nucleocapsid. Subsequently, Co-IP and co-localization experiments confirmed the interaction between the host Myosin 1B (MYO1B) protein and the C protein. Key interaction sites were identified by generating and analyzing various C protein point mutations and truncation variants. The results indicate that specific sites at the N-terminus of the C protein significantly impact its interaction with MYO1B. Ultimately, by modulating MYO1B expression, we found that MYO1B knockdown significantly reduced the C protein and viral genome content in exosomes, leading to a decrease in CSFV titers. These findings underscore the critical role of MYO1B in facilitating the transport of the C protein and viral genome into exosomes during CSFV infection. Overall, this study explores the mechanism of infectious exosome formation during CSFV infection, revealing the critical role of the host MYO1B in this process. This is the first study to identify the involvement of MYO1B in viral infection, not only offering important insights into host-virus interactions but also identifying a new target for antiviral drug development.
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