Anti-Inflammatory and Antioxidant Properties of Squalene in Copper Sulfate-Induced Inflammation in Zebrafish (Danio rerio)
文献类型： 外文期刊
作者： Zhang, Peng 1 ; Liu, Naicheng 1 ; Xue, Mingyang 1 ; Zhang, Mengjie 1 ; Xiao, Zidong 1 ; Xu, Chen 1 ; Fan, Yuding 1 ; Liu, Wei 1 ; Qiu, Junqiang 2 ; Zhang, Qinghua 2 ; Zhou, Yong 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China
2.Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai 201306, Peoples R China
3.Shanghai Ocean Univ, Natl Pathogen Collect Ctr Aquat Anim, Shanghai 201306, Peoples R China
关键词： squalene; zebrafish (Danio rerio); CuSO4; anti-inflammation; antioxidation
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN： 1661-6596
年卷期： 2023 年 24 卷 10 期
页码：
收录情况： SCI
摘要： Long-term or excessive oxidative stress can cause serious damage to fish. Squalene can be added to feed as an antioxidant to improve the body constitution of fish. In this study, the antioxidant activity was detected by 2,2-diphenyl-1-acrylhydrazyl (DPPH) test and fluorescent probe (dichloro-dihydro-fluorescein diacetate). Transgenic Tg (lyz: DsRed2) zebrafish were used to evaluate the effect of squalene on CuSO4-induced inflammatory response. Quantitative real-time reverse transcription polymerase chain reaction was used to examine the expression of immune-related genes. The DPPH assay demonstrated that the highest free radical scavenging exerted by squalene was 32%. The fluorescence intensity of reactive oxygen species (ROS) decreased significantly after 0.7% or 1% squalene treatment, and squalene could exert an antioxidative effect in vivo. The number of migratory neutrophils in vivo was significantly reduced after treatment with different doses of squalene. Moreover, compared with CuSO4 treatment alone, treatment with 1% squalene upregulated the expression of sod by 2.5-foldand gpx4b by 1.3-fold to protect zebrafish larvae against CuSO4-induced oxidative damage. Moreover, treatment with 1% squalene significantly downregulated the expression of tnfa and cox2. This study showed that squalene has potential as an aquafeed additive to provide both anti-inflammatory and antioxidative properties.
- 相关文献
作者其他论文 更多>>
-
Rhein: A potent immunomodulator empowering largemouth bass against MSRV infection
作者：Zhang, Xu;Liu, Lei;Wang, Huan;Qiu, Tianxiu;Zhou, Yan;Shan, Lipeng;Wang, Zixuan;Hu, Yang;Chen, Jiong;Zhang, Xu;Liu, Lei;Wang, Huan;Qiu, Tianxiu;Zhou, Yan;Shan, Lipeng;Wang, Zixuan;Hu, Yang;Chen, Jiong;Xue, Mingyang;Liu, Guanglu
关键词：Rhein; Immunomodulator; Largemouth bass; Apoptosis; Micropterus salmoides rhabdovirus; Antiviral
-
Effects of dietary microbial protease on growth performance, nutrient apparent digestibility, hepatic antioxidant capacity, protease activities and intestinal microflora in juvenile genetically improved farmed tilapia, Oreochromis niloticus
作者：Wu, Jianjun;Wang, Qijun;Zhang, Chengjie;Fu, Dabo;Xu, Li;Zhou, Ying;Yu, Ting;Liu, Wei;Jiang, Ming;Wu, Jinping;Wu, Jinping
关键词：Different proteases; Growth performance; Apparent digestibility; Protease activities; Intestine microbiota
-
Hypolipidemic Effect of Rice Bran Oil Extract Tocotrienol in High-Fat Diet-Induced Hyperlipidemia Zebrafish (Danio Rerio) Induced by High-Fat Diet
作者：Liu, Naicheng;Zhang, Peng;Xue, Mingyang;Zhang, Mengwei;Huang, Zhenyu;Xu, Chen;Meng, Yan;Fan, Yuding;Liu, Wei;Zhang, Feixiang;Zhou, Yong;Zhang, Feixiang;Chen, Peng
关键词：rice bran oil extract; tocotrienol (T3); zebrafish; type 2 diabetes mellitus (T2DM) model; high-fat diet; blood fat
-
Exploring the Effects of Nano-CaCO3 on the Core-Shell Structure and Properties of HDPE/POE/Nano-CaCO3 Ternary Nanocomposites
作者：Liu, Wei;Wang, Lumin;Zhang, Xun;Huang, Hongliang;Liu, Yongli;Min, Minghua;Min, Minghua
关键词：core-shell structure; HDPE; POE; nano-CaCO3; ternary nanocomposites; mechanical properties
-
Isolation and characterization of Bacillus cereus virulent phage CA1
作者：Xiao, Zidong;Hu, Xiaowei;Xue, Mingyang;Jiang, Nan;Fan, Yuding;Zhou, Yong;Hu, Xiaowei;Kong, Xianghui;Chen, Jingtao;Zhang, Chunjie;Liu, Xiaodan
关键词：Bacillus cereus; Chinese soft-shell turtle; Phages; Characterization; Whole genome sequencing analysis
-
The Effects of Dietary n-3 Highly Unsaturated Fatty Acids on Growth, Antioxidant Capacity, Immunity, and Oxylipin Profiles in Acipenser dabryanus
作者：Wu, Jinping;Xiong, Wei;Liu, Wei;Wu, Jinming;Ruan, Rui;Wang, Yuqi;Liu, Yuan;Leng, Xiaoqian;Li, Pengcheng;Zhong, Jia;Du, Hao;Fu, Peng;Zhang, Chuang
关键词：Acipenser dabryanus; dietary n-3 HUFA levels; growth performance; antioxidant; immunity capacity; serum oxylipin
-
β-Sitosterol Reduces the Content of Triglyceride and Cholesterol in a High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease Zebrafish (Danio rerio) Model
作者：Zhang, Peng;Liu, Naicheng;Xue, Mingyang;Zhang, Mengjie;Xiao, Zidong;Xu, Chen;Fan, Yuding;Zhou, Yong;Zhang, Peng;Liu, Naicheng;Zhang, Mengjie;Qiu, Junqiang;Zhang, Qinghua;Zhang, Peng;Liu, Naicheng;Zhang, Mengjie;Qiu, Junqiang;Zhang, Qinghua
关键词：beta-sitosterol; non-alcoholic fatty liver disease model; zebrafish; high-fat diet; blood fat untargeted lipidomics; lipid metabolism
